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• W2974604132 abstract "Abstract Recent advances in fluorogen-binding “light-up” RNA aptamers have enabled protein-free detection of RNA in cells. Detailed biophysical characterization of folding of G-Quadruplex (GQ)-based light-up aptamers such as Spinach, Mango and Corn is still lacking despite the potential implications on their folding and function. In this work we employ single-molecule fluorescence-force spectroscopy to examine mechanical responses of Spinach2, i MangoIII and MangoIV. Spinach2 unfolds in four discrete steps as force is increased to 7 pN and refolds in reciprocal steps upon force relaxation. In contrast, GQ-core unfolding in i MangoIII and MangoIV occurs in one discrete step at forces >10 pN and refolding occurred at lower forces showing hysteresis. Co-transcriptional folding using superhelicases shows reduced misfolding propensity and allowed a folding pathway different from refolding. Under physiologically relevant pico-Newton levels of force, these aptamers may unfold in vivo and subsequently misfold. Understanding of the dynamics of RNA aptamers will aid engineering of improved fluorogenic modules for cellular applications." @default.
• W2974604132 created "2019-09-26" @default.
• W2974604132 creator A5010272295 @default.
• W2974604132 creator A5026059874 @default.
• W2974604132 date "2019-09-20" @default.
• W2974604132 modified "2023-09-30" @default.
• W2974604132 title "Nanomechanics and co-transcriptional folding of Spinach and Mango" @default.
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• W2974604132 doi "https://doi.org/10.1038/s41467-019-12299-y" @default.
• W2974604132 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6754394" @default.
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• W2974604132 hasPublicationYear "2019" @default.
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