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- W2975008256 abstract "Plaque assay has been used for a long time to determine infectious titers and characterize prokaryotic and eukaryotic viruses forming plaques. Indeed, plaque morphology and dimensions can provide information regarding the replication kinetics and the virulence of a particular virus. In this work, we present ViralPlaque, a fast, open-source and versatile ImageJ macro for the automated determination of viral plaque dimensions from digital images. Also, a machine learning plugin is integrated in the analysis algorithm for adaptation of ViralPlaque to the user’s needs and experimental conditions. A high correlation between manual and automated measurements of plaque dimensions was demonstrated. This macro will facilitate reliable and reproducible characterization of cytolytic viruses with an increased processing speed." @default.
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- W2975008256 date "2019-09-24" @default.
- W2975008256 modified "2023-10-10" @default.
- W2975008256 title "ViralPlaque: a Fiji macro for automated assessment of viral plaque statistics" @default.
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- W2975008256 doi "https://doi.org/10.7717/peerj.7729" @default.
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