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• W2975106862 abstract "Abstract Previously, we have indicated that Hot Water Extract (HWE) from Spent Mushroom Substrate (SMS) of Ganoderma lucidum enhanced immune function of normal mice, and improved antioxidant activity and enhanced immune function of immunosuppression mice induced by cyclophosphamide (Cy). Here we performed the high throughput RNA sequencing strategy using Illumina HiSeq™ 2000 to characterize the spleen transcriptome from normal (CK1), HWE-treated (CK2), Cy-treated (CY) and both high dose HWE and Cy-treated mice (CH). From the RNA Sequencing, total mapped reads of map to Gene in CK1, CK2, CY and CH was 54 759 942, 54 678 926, 44 728 132 and 54 006 596, respectively. And gene expression was significantly different among CK1 and CK2, CY and CH. Compared with CK1, the gene expression of Ugt1a6b was down-regulated in CK2 after HWE treated. In addition, compared with CY, multiple tumor suppressor or tumorigenesis genes were down-regulated, such as Cdkn1a , Cdkn1b , Mapk 10, Vash1 , and Tnc and other genes in CK2 and CH. Taken together, our study highlighted the spleen transcriptome profiles of C57BL/6 mouse in response to HWE from SMS of G. lucidum and Cy, and indicated that HWE can improve the immune function of the mouse and accelerated the recovery of immunosuppression in Cy-treated mice." @default.
• W2975106862 created "2019-10-03" @default.
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• W2975106862 date "2019-09-26" @default.
• W2975106862 modified "2023-09-28" @default.
• W2975106862 title "Spleen Transcriptome Analysis of C57BL/6 Mouse in Response to Hot Water Extract from Spent Mushroom Substrate of Ganoderma lucidum and Cyclophosphamide" @default.
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• W2975106862 doi "https://doi.org/10.1101/784108" @default.
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