FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2975402758> ?p ?o ?g. }

• W2975402758 endingPage "222" @default.
• W2975402758 startingPage "213" @default.
• W2975402758 abstract "Current 3D culture models to study colorectal cancer lack architectural support and signaling proteins provided by the tissue extracellular matrix (ECM) which may influence cell behavior and cancer progression. Therefore, the ability to study cancer cells in the context of a matrix that is physiologically more relevant and to understand how the ECM affects cancer progression has been understudied. To address this, we developed an ex-vivo 3D system, provided by intact wild type (WT) and colon cancer susceptible decellularized mouse colons (DMC), to support the growth of human cancer cells. DMC are free of viable cells but still contain extracellular matrix proteins including subsets of collagens. Stiffness, an important mechanical property, is also maintained in DMCs. Importantly, we observed that the DMC is permissive for cell proliferation and differentiation of a human colon cancer cell line (HT-29). Notably, the ability of cells in the WT DMC to differentiate was also greater when compared to Matrigel™, an extracellular matrix extract from a mouse tumor cell line. Additionally, we observed in invasion assays that DMC obtained from polyps from a colon cancer susceptible mouse model facilitated increased cell migration/invasion of colorectal cancer cells and immortalized non-tumor colonic epithelial cells compared to DMC from WT mice. Finally, using mass spectrometry, we identified extracellular matrix proteins that are more abundant in DMC from a colorectal cancer mouse model compared to age and sex-matched WT mice. We propose that these abundantly expressed proteins in the tumor microenvironment are potentially involved in colorectal cancer progression. Decellularized matrices, when properly produced, are attractive biomaterials for tissue regeneration and replacement. We show here that the mouse decellularized matrices can also be repurposed to elucidate how the extracellular matrix influences human cell behavior and cancer progression. To do this we produce decellularized matrices, from mice colonic tissue, that have preserved tissue mechanical and structural properties. We demonstrate that the matrix better supports the differentiation of HT-29 cells, a colonic cancer cell line, compared to Matrigel™. Additionally, we show that the extracellular matrix contributes to colon cancer progression via invasion assays using extracellular matrix extracts. Finally, we use mass spectrometry to identify ECM proteins that are more abundant in colonic polyps compared to adjacent tissue regions. This model system may have therapeutic implications for colorectal cancer patients." @default.
• W2975402758 created "2019-10-03" @default.
• W2975402758 creator A5037254224 @default.
• W2975402758 creator A5045374495 @default.
• W2975402758 creator A5081012778 @default.
• W2975402758 date "2019-12-01" @default.
• W2975402758 modified "2023-10-16" @default.
• W2975402758 title "Decellularized mice colons as models to study the contribution of the extracellular matrix to cell behavior and colon cancer progression" @default.
• W2975402758 cites W1862989971 @default.
• W2975402758 cites W1912159405 @default.
• W2975402758 cites W1968769948 @default.
• W2975402758 cites W1981521674 @default.
• W2975402758 cites W1981778940 @default.
• W2975402758 cites W1989696897 @default.
• W2975402758 cites W1991201011 @default.
• W2975402758 cites W2005526299 @default.
• W2975402758 cites W2017615910 @default.
• W2975402758 cites W2023026872 @default.
• W2975402758 cites W2031111161 @default.
• W2975402758 cites W2034269086 @default.
• W2975402758 cites W2035516992 @default.
• W2975402758 cites W2038078260 @default.
• W2975402758 cites W2039628059 @default.
• W2975402758 cites W2045295282 @default.
• W2975402758 cites W2046338893 @default.
• W2975402758 cites W2051028769 @default.
• W2975402758 cites W2057009728 @default.
• W2975402758 cites W2059486645 @default.
• W2975402758 cites W2066523597 @default.
• W2975402758 cites W2071184715 @default.
• W2975402758 cites W2086715657 @default.
• W2975402758 cites W2103883318 @default.
• W2975402758 cites W2107734506 @default.
• W2975402758 cites W2116379215 @default.
• W2975402758 cites W2118417553 @default.
• W2975402758 cites W2132566055 @default.
• W2975402758 cites W2156358891 @default.
• W2975402758 cites W2174570955 @default.
• W2975402758 cites W2203252340 @default.
• W2975402758 cites W2334652946 @default.
• W2975402758 cites W2545703737 @default.
• W2975402758 cites W2546877613 @default.
• W2975402758 cites W2552754421 @default.
• W2975402758 cites W2609961151 @default.
• W2975402758 cites W2736722603 @default.
• W2975402758 cites W2738450069 @default.
• W2975402758 cites W2748612199 @default.
• W2975402758 cites W2763398087 @default.
• W2975402758 cites W2769412673 @default.
• W2975402758 cites W2800878807 @default.
• W2975402758 cites W2801004279 @default.
• W2975402758 cites W2904720151 @default.
• W2975402758 cites W2952948597 @default.
• W2975402758 cites W391925564 @default.
• W2975402758 doi "https://doi.org/10.1016/j.actbio.2019.09.033" @default.
• W2975402758 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31562987" @default.
• W2975402758 hasPublicationYear "2019" @default.
• W2975402758 type Work @default.
• W2975402758 sameAs 2975402758 @default.
• W2975402758 citedByCount "15" @default.
• W2975402758 countsByYear W29754027582020 @default.
• W2975402758 countsByYear W29754027582021 @default.
• W2975402758 countsByYear W29754027582022 @default.
• W2975402758 countsByYear W29754027582023 @default.
• W2975402758 crossrefType "journal-article" @default.
• W2975402758 hasAuthorship W2975402758A5037254224 @default.
• W2975402758 hasAuthorship W2975402758A5045374495 @default.
• W2975402758 hasAuthorship W2975402758A5081012778 @default.
• W2975402758 hasBestOaLocation W29754027581 @default.
• W2975402758 hasConcept C121608353 @default.
• W2975402758 hasConcept C185592680 @default.
• W2975402758 hasConcept C189165786 @default.
• W2975402758 hasConcept C190672674 @default.
• W2975402758 hasConcept C2776107976 @default.
• W2975402758 hasConcept C2778608917 @default.
• W2975402758 hasConcept C2779256057 @default.
• W2975402758 hasConcept C2780394083 @default.
• W2975402758 hasConcept C502942594 @default.
• W2975402758 hasConcept C526805850 @default.
• W2975402758 hasConcept C54355233 @default.
• W2975402758 hasConcept C81885089 @default.
• W2975402758 hasConcept C86803240 @default.
• W2975402758 hasConcept C95444343 @default.
• W2975402758 hasConcept C96232424 @default.
• W2975402758 hasConceptScore W2975402758C121608353 @default.
• W2975402758 hasConceptScore W2975402758C185592680 @default.
• W2975402758 hasConceptScore W2975402758C189165786 @default.
• W2975402758 hasConceptScore W2975402758C190672674 @default.
• W2975402758 hasConceptScore W2975402758C2776107976 @default.
• W2975402758 hasConceptScore W2975402758C2778608917 @default.
• W2975402758 hasConceptScore W2975402758C2779256057 @default.
• W2975402758 hasConceptScore W2975402758C2780394083 @default.
• W2975402758 hasConceptScore W2975402758C502942594 @default.
• W2975402758 hasConceptScore W2975402758C526805850 @default.
• W2975402758 hasConceptScore W2975402758C54355233 @default.
• W2975402758 hasConceptScore W2975402758C81885089 @default.
• W2975402758 hasConceptScore W2975402758C86803240 @default.
• W2975402758 hasConceptScore W2975402758C95444343 @default.

• next