FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2975705087> ?p ?o ?g. }

• W2975705087 endingPage "13" @default.
• W2975705087 startingPage "13" @default.
• W2975705087 abstract "In 2004, a vein yellowing disease of soil-grown greenhouse capsicum was observed in southern Australia (Fig. 1). Symptoms were similar to those of pepper yellow vein disease, a suspected viral disease of capsicum transmitted by soilborne Olpidium species (Fletcher et al., 1). Disease symptoms were apparent on young plants and faded as the plant matured. In 2008, the disease was again detected in this region and was graft-transmitted to four capsicum plants which developed vein-yellowing symptoms. This isolate (2155) and a sample lyophilised in 2004 (isolate 1631) were tested by RT-PCR using the OP1 and OP2 primers specific for RNA-1 of the genus Ophiovirus (Varia et al., 4). RT-PCR amplicons of the expected size (136 bp) were obtained from the suspected virus-infected samples but not from healthy capsicum. A larger amplicon was amplified using the OP2 primer and a newly designed degenerate genus-specific primer OP3 (5‘-TCDCAAACHCAAGTACAAATGGAAG-3‘) in RT-PCR. The product was amplified from isolate 1631 and the amplicons cloned. Four clones were sequenced, and all were identical (GenBank Accesssion No. MN128532). This sequence was 97.5% identical to that of Ranunculus white mottle virus (RWMV, AF335429) using a pairwise nucleotide sequence alignment. ICTV criteria for classifying Ophiovirus species are based on coat protein sequence (http://www.ictv.global/report/aspiviridae; Garcia et al., 2; 3), however, there are no RWMV coat protein sequences available for comparison. Based on available sequence, the virus is identified as RWMV. Specific primers, RWMVF1 (5'-CGAACATTCCATCTACGCCT-3') and RWMVR1 (5'-GATAGACAATGCCGCAACAA-3') were developed and used in RT-PCR to screen field samples. The expected 383 bp amplicons were obtained from the original two symptomatic field samples collected from southern Australian and samples from the four graft-transmitted plants. In 2018, disease symptoms were seen on capsicum plants in seven greenhouse crops inspected in geographically separated areas of the growing region. The disease incidence was between 1-5% and infection by RWMV was confirmed using the specific RT-PCR in samples collected from these greenhouses. No incidence data is available for the earlier detections. In Italy, RWMV was reported to infect ranunculus (Ranunculus asiaticus hybrids) (Vaira et al., 5) and anemone (Anemone coronaria) (Vaira et al., 6) and was mechanically transmitted from Nicotiana benthamiana to both N. clevelandii and N. megalosiphon where it elicited systemic infection (Vaira et al., 5). There is no evidence that RWMV causes significant economic loss in capsicum crops, but symptoms can be confused with those caused by the more damaging Tomato spotted wilt virus which can lead to inappropriate management choices. This is the first report of RWMV in Australia, and of the virus infecting capsicum. It is, however, likely that RWMV is the cause of the earlier reports of pepper yellow vein disease in this host (Fletcher et al., 1). We thank growers for access to plants and Hort Innovation Australia, the Australian vegetable industry and Australian Federal Government for funding." @default.
• W2975705087 created "2019-10-03" @default.
• W2975705087 creator A5033881393 @default.
• W2975705087 creator A5046355229 @default.
• W2975705087 creator A5091029157 @default.
• W2975705087 date "2019-07-01" @default.
• W2975705087 modified "2023-10-03" @default.
• W2975705087 title "First record of <i>Ranunculus white mottle virus</i> from Australia" @default.
• W2975705087 cites W2003985248 @default.
• W2975705087 cites W2039070344 @default.
• W2975705087 cites W2078788837 @default.
• W2975705087 cites W2110346056 @default.
• W2975705087 cites W2642118967 @default.
• W2975705087 cites W2810168904 @default.
• W2975705087 doi "https://doi.org/10.5197/j.2044-0588.2019.040.013" @default.
• W2975705087 hasPublicationYear "2019" @default.
• W2975705087 type Work @default.
• W2975705087 sameAs 2975705087 @default.
• W2975705087 citedByCount "3" @default.
• W2975705087 countsByYear W29757050872022 @default.
• W2975705087 countsByYear W29757050872023 @default.
• W2975705087 crossrefType "journal-article" @default.
• W2975705087 hasAuthorship W2975705087A5033881393 @default.
• W2975705087 hasAuthorship W2975705087A5046355229 @default.
• W2975705087 hasAuthorship W2975705087A5091029157 @default.
• W2975705087 hasBestOaLocation W29757050871 @default.
• W2975705087 hasConcept C104317684 @default.
• W2975705087 hasConcept C109110057 @default.
• W2975705087 hasConcept C141231307 @default.
• W2975705087 hasConcept C159047783 @default.
• W2975705087 hasConcept C178790620 @default.
• W2975705087 hasConcept C185592680 @default.
• W2975705087 hasConcept C20850961 @default.
• W2975705087 hasConcept C2522874641 @default.
• W2975705087 hasConcept C2777563447 @default.
• W2975705087 hasConcept C2780951447 @default.
• W2975705087 hasConcept C2781191690 @default.
• W2975705087 hasConcept C49105822 @default.
• W2975705087 hasConcept C54355233 @default.
• W2975705087 hasConcept C59822182 @default.
• W2975705087 hasConcept C79029880 @default.
• W2975705087 hasConcept C8185291 @default.
• W2975705087 hasConcept C86803240 @default.
• W2975705087 hasConceptScore W2975705087C104317684 @default.
• W2975705087 hasConceptScore W2975705087C109110057 @default.
• W2975705087 hasConceptScore W2975705087C141231307 @default.
• W2975705087 hasConceptScore W2975705087C159047783 @default.
• W2975705087 hasConceptScore W2975705087C178790620 @default.
• W2975705087 hasConceptScore W2975705087C185592680 @default.
• W2975705087 hasConceptScore W2975705087C20850961 @default.
• W2975705087 hasConceptScore W2975705087C2522874641 @default.
• W2975705087 hasConceptScore W2975705087C2777563447 @default.
• W2975705087 hasConceptScore W2975705087C2780951447 @default.
• W2975705087 hasConceptScore W2975705087C2781191690 @default.
• W2975705087 hasConceptScore W2975705087C49105822 @default.
• W2975705087 hasConceptScore W2975705087C54355233 @default.
• W2975705087 hasConceptScore W2975705087C59822182 @default.
• W2975705087 hasConceptScore W2975705087C79029880 @default.
• W2975705087 hasConceptScore W2975705087C8185291 @default.
• W2975705087 hasConceptScore W2975705087C86803240 @default.
• W2975705087 hasIssue "1" @default.
• W2975705087 hasLocation W29757050871 @default.
• W2975705087 hasLocation W29757050872 @default.
• W2975705087 hasOpenAccess W2975705087 @default.
• W2975705087 hasPrimaryLocation W29757050871 @default.
• W2975705087 hasRelatedWork W2043999806 @default.
• W2975705087 hasRelatedWork W2054377249 @default.
• W2975705087 hasRelatedWork W2125155197 @default.
• W2975705087 hasRelatedWork W2363798439 @default.
• W2975705087 hasRelatedWork W2415272311 @default.
• W2975705087 hasRelatedWork W2975705087 @default.
• W2975705087 hasRelatedWork W303927472 @default.
• W2975705087 hasRelatedWork W3111028746 @default.
• W2975705087 hasRelatedWork W3136981226 @default.
• W2975705087 hasRelatedWork W4379966686 @default.
• W2975705087 hasVolume "40" @default.
• W2975705087 isParatext "false" @default.
• W2975705087 isRetracted "false" @default.
• W2975705087 magId "2975705087" @default.
• W2975705087 workType "article" @default.