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- W2975710904 abstract "To investigate the therapeutic potential for chronic pain by recombinant adenorivus(Ad) expressing human beta-nerve growth factor (hNGF#beta#). The gene of interest hNGF#beta# was inserted into the XmaⅠ-XbaⅠsite of pBluescriptⅡSK (+) plasmid to form the plasmid of pBluescript ⅡSK (+)-hNGF#beta#. The hNGF#beta# cDNA was digested from pBluescript ⅡSK (+)-hNGF#beta#, then subcloned into the shuttle plasmid of pShuttle-CMV and formed transfer plasmid of pShuttle-CMV-hNGF#beta#. pShuttle-CMV-hNGF#beta# was transformed into the prepared ultracompetent BJ5183 containing pAdEasy-1 to construct the recombinant of hNGF#beta# adenoviral plasmid. pAdeasy-1-hNGF#beta# was transfected into AD293 cells with Lipovec, and Ad-hNGF#beta# was packaged within AD293 cells. CR test and gene sequencing indicated that the recombinant adenovirus plasmid pAdEasy-1-hNGF#beta# and Ad-hNGF#beta# contained NGF cDNA. The homologous recombination in bacteria is a convenient and efficient method to prepare recombinant adenovirus plasmid, pAdEasy-1-hNGF#beta# and Ad-hNGF#beta# were successfully prepared. This research provides a basis for the pain management with hNGF#beta# gene therapy." @default.
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- W2975710904 date "2005-01-01" @default.
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- W2975710904 title "Construction of aecombinant human beta-nerve growth factor adenorivus by homologous recombination in bacteria" @default.
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