Matches in SemOpenAlex for { <https://semopenalex.org/work/W2976179930> ?p ?o ?g. }
- W2976179930 endingPage "168" @default.
- W2976179930 startingPage "158" @default.
- W2976179930 abstract "SIRT6, a member of the sirtuin family, is a NAD + dependent protein deacetylase and has been implicated in transcriptional regulation of somatic cells and post-transcriptional regulation of oocyte meiosis. However, the function of cumulus cell-derived and maternal SIRT6 in meiotic maturation of porcine oocytes is not yet known. Here, we report that SIRT6 mRNA and protein exists in the oocyte and its surrounding cumulus cells during meiotic maturation. Functional studies using a specific inhibitor in cumulus-enclosed oocytes revealed important roles for SIRT6 in germinal vesicle breakdown (GVBD) and cumulus expansion. Moreover, inhibitor treatment led to a significant reduction in the rate of first polar body (PB1) extrusion and early development of parthenogenetically activated embryos. In contrast, SIRT6 inhibition in cumulus-free oocytes only resulted in a significant reduction in the rate of PB1 extrusion. Furthermore, SIRT6 dysfunction regardless of the origin in both cumulus cells and oocytes severely impaired spindle organization and chromosome alignment at the metaphase stage. Molecularly, SIRT6 inhibition in cumulus cells significantly reduced expression of genes associated with cumulus expansion and gap junctional communication and even expression levels of active phosphorylated CDK1 in oocytes. Importantly, adenylate cyclase inhibition could partially rescue GVBD and PB1 extrusion in SIRT6-inhibited cumulus-enclosed oocytes. Taken together, these results demonstrate that cumulus cell-expressed and maternal SIRT6 differentially regulates porcine oocyte meiotic maturation." @default.
- W2976179930 created "2019-10-03" @default.
- W2976179930 creator A5016702851 @default.
- W2976179930 creator A5034261756 @default.
- W2976179930 creator A5038160567 @default.
- W2976179930 creator A5043415956 @default.
- W2976179930 creator A5044805857 @default.
- W2976179930 creator A5046145976 @default.
- W2976179930 creator A5065652716 @default.
- W2976179930 creator A5072796155 @default.
- W2976179930 creator A5076642314 @default.
- W2976179930 creator A5083228843 @default.
- W2976179930 creator A5085420366 @default.
- W2976179930 date "2020-01-01" @default.
- W2976179930 modified "2023-10-09" @default.
- W2976179930 title "Cumulus cell-derived and maternal SIRT6 differentially regulates porcine oocyte meiotic maturation" @default.
- W2976179930 cites W1530826550 @default.
- W2976179930 cites W1830081733 @default.
- W2976179930 cites W1893337122 @default.
- W2976179930 cites W1981231686 @default.
- W2976179930 cites W1986462100 @default.
- W2976179930 cites W1994540097 @default.
- W2976179930 cites W1994992498 @default.
- W2976179930 cites W2011979646 @default.
- W2976179930 cites W2012400528 @default.
- W2976179930 cites W2032325024 @default.
- W2976179930 cites W2042716686 @default.
- W2976179930 cites W2046716387 @default.
- W2976179930 cites W2054866841 @default.
- W2976179930 cites W2056555106 @default.
- W2976179930 cites W2095849729 @default.
- W2976179930 cites W2105702352 @default.
- W2976179930 cites W2109560454 @default.
- W2976179930 cites W2111155686 @default.
- W2976179930 cites W2116761578 @default.
- W2976179930 cites W2120956575 @default.
- W2976179930 cites W2123961466 @default.
- W2976179930 cites W2129349115 @default.
- W2976179930 cites W2145620343 @default.
- W2976179930 cites W2147062511 @default.
- W2976179930 cites W2168761262 @default.
- W2976179930 cites W2340936863 @default.
- W2976179930 cites W2343664903 @default.
- W2976179930 cites W2345417751 @default.
- W2976179930 cites W2590240788 @default.
- W2976179930 cites W2624644197 @default.
- W2976179930 cites W2725322436 @default.
- W2976179930 cites W2758027132 @default.
- W2976179930 cites W2765597179 @default.
- W2976179930 cites W2784544796 @default.
- W2976179930 cites W2806355679 @default.
- W2976179930 cites W2808731361 @default.
- W2976179930 cites W2899058437 @default.
- W2976179930 cites W2913012073 @default.
- W2976179930 cites W2915726494 @default.
- W2976179930 cites W2921018061 @default.
- W2976179930 doi "https://doi.org/10.1016/j.theriogenology.2019.09.048" @default.
- W2976179930 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31593883" @default.
- W2976179930 hasPublicationYear "2020" @default.
- W2976179930 type Work @default.
- W2976179930 sameAs 2976179930 @default.
- W2976179930 citedByCount "12" @default.
- W2976179930 countsByYear W29761799302021 @default.
- W2976179930 countsByYear W29761799302022 @default.
- W2976179930 countsByYear W29761799302023 @default.
- W2976179930 crossrefType "journal-article" @default.
- W2976179930 hasAuthorship W2976179930A5016702851 @default.
- W2976179930 hasAuthorship W2976179930A5034261756 @default.
- W2976179930 hasAuthorship W2976179930A5038160567 @default.
- W2976179930 hasAuthorship W2976179930A5043415956 @default.
- W2976179930 hasAuthorship W2976179930A5044805857 @default.
- W2976179930 hasAuthorship W2976179930A5046145976 @default.
- W2976179930 hasAuthorship W2976179930A5065652716 @default.
- W2976179930 hasAuthorship W2976179930A5072796155 @default.
- W2976179930 hasAuthorship W2976179930A5076642314 @default.
- W2976179930 hasAuthorship W2976179930A5083228843 @default.
- W2976179930 hasAuthorship W2976179930A5085420366 @default.
- W2976179930 hasConcept C104317684 @default.
- W2976179930 hasConcept C119157956 @default.
- W2976179930 hasConcept C134305767 @default.
- W2976179930 hasConcept C148196450 @default.
- W2976179930 hasConcept C16685009 @default.
- W2976179930 hasConcept C185592680 @default.
- W2976179930 hasConcept C196843134 @default.
- W2976179930 hasConcept C201604863 @default.
- W2976179930 hasConcept C2776690073 @default.
- W2976179930 hasConcept C2777595374 @default.
- W2976179930 hasConcept C2780561631 @default.
- W2976179930 hasConcept C2994119143 @default.
- W2976179930 hasConcept C54355233 @default.
- W2976179930 hasConcept C71924100 @default.
- W2976179930 hasConcept C86803240 @default.
- W2976179930 hasConcept C95444343 @default.
- W2976179930 hasConceptScore W2976179930C104317684 @default.
- W2976179930 hasConceptScore W2976179930C119157956 @default.
- W2976179930 hasConceptScore W2976179930C134305767 @default.
- W2976179930 hasConceptScore W2976179930C148196450 @default.
- W2976179930 hasConceptScore W2976179930C16685009 @default.