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• W2976281633 abstract "Background Long-term implant success has been shown to be related to the presence of a soft tissue barrier, which prevents bacterial penetration. The quality of this mucosal attachment is influenced by the properties of the implant components that are placed in contact with the soft tissue. Although, different study has been performed there is still lack of information on which is the implant surface that better promote the soft tissue cells adhesion. Aim/Hypothesis The aim of the present study is to evaluate the adhesion of human soft tissues surrounding a custom-made healing abutments. Material and Methods A total of 21 healing abutments with alternate laser-conditioned and machined surfaces with attached the gingival biopsies have been collected after 4 weeks of healing. The ìG.d'Annunzioî Chieti-Pescara University Ethic Committee has approved this clinical trial N°22 of 18.10.2018 Paraffin sections of human gingiva surrounding the healing abutments were prepared from 10 subjects. Hematoxylin Eosin and Trichrome staining and image analysis were performed to measure and compare the morphological differences among junctional epithelium, sulcular epithelium and the subepithelial connective tissue of the gingiva touching the smooth and the laser-conditioned surface of the healing abutment. May-Grunwald Giemsa staining and immunofluorescence for CD63 were performed to identify inflammation area. Results The region of the gingiva adherent to the laser-conditioned showed a regular morphology in all its parts (junctional epithelium, sulcular epithelium and subepithelial connective tissue). By contrast, the region of the gingiva adherent to the machined surfaces showed several stress hallmarks-a) disruption and discontinuities in the basal membrane associated with areas of increased inflammation evident both in the epithelium and, in a larger extent, in the subepithelial connective (abundant presence of CD63 positive area that is surface marker specific for neutrophils present in inflamed tissue and it is not expressed by neutrophils present in healthy tissue).b) The correct keratinization process is defective or absent since the corneum status present still nucleated cells or a very thin enucleated cells layer compared to the regular corneal layer of the epithelium of the part of gingiva facing the laser-conditioned surface Conclusion and Clinical Implications This data suggest that the use of laser treated surface on healing abutment could positively influence the healing. A better distribution and cell morphology on test surface could encourage the clinical use of this treatment. The laser treated surface seems to influence the cell adhesion and the inflammatory infiltrate of the soft tissue. However, further study will be necessary to better understand biological aspects related to soft tissue adhesion on abutment and prosthetic materials." @default.
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• W2976281633 date "2019-09-01" @default.
• W2976281633 modified "2023-10-17" @default.
• W2976281633 title "Preliminary results of human soft tissues adhesion around different treated healing abutments" @default.
• W2976281633 doi "https://doi.org/10.1111/clr.150_13509" @default.
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