Matches in SemOpenAlex for { <https://semopenalex.org/work/W2976299017> ?p ?o ?g. }
- W2976299017 abstract "Abstract Persimmon proanthocyanidin (PA) biosynthetic had been reported to be regulated by several transcription factors, but the miRNAs function involved in this process was poorly understood. We identified a miRNA858b that putatively targeted two R2R3-MYB transcription factors, DkMYB19 / DkMYB20 . Transcript accumulation of DkMYB19 / DkMYB20 and miRNA858b showed contrasting divergent expression patterns during fruit development. DkMYB19/DkMYB20 were confirmed to be localized in the nucleus. The interaction between miRNA858b and DkMYB19 / DkMYB20 were experimentally validated by 5’ RNA ligase-mediated RACE and LUC enzyme activity detection. Overexpression of miRNA858b led to the down-regulation of DkMYB19 / DkMYB20 which reduced the accumulation of PA, whereas the reduced miRNA858b activity that up-regulated the DkMYB19 / DkMYB20 resulted in high levels of PA in STTM858b transient expression in leaves in vivo . Similarly, the transient transformation of miRNA858b in fruit wafers in vitro also reduced the accumulation of PA by repressing the DkMYB19 / DkMYB20 , while the up-regulation of DkMYB19 / DkMYB20 enhanced the accumulation of PA in STTM858b or DkMYB19 / DkMYB20 transient transformation in fruit wafers. PA content decreased after overexpression of miRNA858b in Arabidopsis wild type and DkMYB19 / DkMYB20 in persimmon leaf callus consisted with the above results. These findings suggested that miRNA858b repressed the expression of DkMYB19 / DkMYB20 which contribute to PA accumulation in persimmon." @default.
- W2976299017 created "2019-10-03" @default.
- W2976299017 creator A5016660156 @default.
- W2976299017 creator A5035555253 @default.
- W2976299017 creator A5054748492 @default.
- W2976299017 creator A5071231459 @default.
- W2976299017 creator A5074140059 @default.
- W2976299017 date "2019-09-29" @default.
- W2976299017 modified "2023-09-27" @default.
- W2976299017 title "miRNA858b Inhibits Proanthocyanidin Accumulation by Repression of DkMYB19 and DkMYB20 in Persimmon" @default.
- W2976299017 cites W1218459554 @default.
- W2976299017 cites W1919318555 @default.
- W2976299017 cites W1944597845 @default.
- W2976299017 cites W1965172979 @default.
- W2976299017 cites W1966859346 @default.
- W2976299017 cites W1969368345 @default.
- W2976299017 cites W1980291963 @default.
- W2976299017 cites W1981276108 @default.
- W2976299017 cites W1982660543 @default.
- W2976299017 cites W1986214186 @default.
- W2976299017 cites W1992743014 @default.
- W2976299017 cites W1993394922 @default.
- W2976299017 cites W1997504763 @default.
- W2976299017 cites W2001058688 @default.
- W2976299017 cites W2003999250 @default.
- W2976299017 cites W2011032227 @default.
- W2976299017 cites W2014752533 @default.
- W2976299017 cites W2025856992 @default.
- W2976299017 cites W2026964616 @default.
- W2976299017 cites W2027033008 @default.
- W2976299017 cites W2037145938 @default.
- W2976299017 cites W2040988226 @default.
- W2976299017 cites W2063910564 @default.
- W2976299017 cites W2064485022 @default.
- W2976299017 cites W2078353600 @default.
- W2976299017 cites W2089007844 @default.
- W2976299017 cites W2092806862 @default.
- W2976299017 cites W2093931964 @default.
- W2976299017 cites W2097145832 @default.
- W2976299017 cites W2097839980 @default.
- W2976299017 cites W2099660828 @default.
- W2976299017 cites W2109341131 @default.
- W2976299017 cites W2117839341 @default.
- W2976299017 cites W2124227746 @default.
- W2976299017 cites W2127446611 @default.
- W2976299017 cites W2132632499 @default.
- W2976299017 cites W2135015580 @default.
- W2976299017 cites W2136797184 @default.
- W2976299017 cites W2139802976 @default.
- W2976299017 cites W2141157874 @default.
- W2976299017 cites W2147380605 @default.
- W2976299017 cites W2147528286 @default.
- W2976299017 cites W2152513532 @default.
- W2976299017 cites W2153149373 @default.
- W2976299017 cites W2159261769 @default.
- W2976299017 cites W2167259011 @default.
- W2976299017 cites W2168909597 @default.
- W2976299017 cites W2169344456 @default.
- W2976299017 cites W2170976550 @default.
- W2976299017 cites W2191917170 @default.
- W2976299017 cites W2281526454 @default.
- W2976299017 cites W2282376110 @default.
- W2976299017 cites W2300721186 @default.
- W2976299017 cites W2313988786 @default.
- W2976299017 cites W2410068980 @default.
- W2976299017 cites W2479545082 @default.
- W2976299017 cites W2537790317 @default.
- W2976299017 cites W2596614460 @default.
- W2976299017 cites W2605243468 @default.
- W2976299017 cites W2766289732 @default.
- W2976299017 cites W2770052934 @default.
- W2976299017 cites W2790462148 @default.
- W2976299017 cites W2806187998 @default.
- W2976299017 cites W2912099069 @default.
- W2976299017 cites W2947219107 @default.
- W2976299017 cites W2947287132 @default.
- W2976299017 cites W3150979592 @default.
- W2976299017 cites W861117788 @default.
- W2976299017 doi "https://doi.org/10.1101/783431" @default.
- W2976299017 hasPublicationYear "2019" @default.
- W2976299017 type Work @default.
- W2976299017 sameAs 2976299017 @default.
- W2976299017 citedByCount "1" @default.
- W2976299017 countsByYear W29762990172020 @default.
- W2976299017 crossrefType "posted-content" @default.
- W2976299017 hasAuthorship W2976299017A5016660156 @default.
- W2976299017 hasAuthorship W2976299017A5035555253 @default.
- W2976299017 hasAuthorship W2976299017A5054748492 @default.
- W2976299017 hasAuthorship W2976299017A5071231459 @default.
- W2976299017 hasAuthorship W2976299017A5074140059 @default.
- W2976299017 hasBestOaLocation W29762990171 @default.
- W2976299017 hasConcept C104317684 @default.
- W2976299017 hasConcept C138885662 @default.
- W2976299017 hasConcept C143065580 @default.
- W2976299017 hasConcept C150194340 @default.
- W2976299017 hasConcept C153911025 @default.
- W2976299017 hasConcept C179926584 @default.
- W2976299017 hasConcept C185592680 @default.
- W2976299017 hasConcept C186310378 @default.
- W2976299017 hasConcept C204241405 @default.