FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2976397575> ?p ?o ?g. }

• W2976397575 endingPage "109953" @default.
• W2976397575 startingPage "109953" @default.
• W2976397575 abstract "Tembusu virus (TMUV) is a contagious pathogen of waterfowl including ducks and geese, which causes symptoms of high fever, loss of appetite and reduced egg production. The development of an effective vaccine is important for the prevention and control of the disease. We evaluated a DNA vaccine based on a recombinant pre-membrane (prM) and envelope (E) protein, using CpG oligodeoxynucleotide (ODN) as an adjuvanted, and tested it for protection efficacy. BHK21 cells were transfected with the recombinant plasmid pVAX1-prM/E-CpG, and the antigenicity of the expressed protein was detected using an indirect immunofluorescence assay (IFA) and western blot assay. One-day-old ducklings were intramuscularly injected with 200 μg doses of pVAX1-prM/E-CpG or pVAX1-CpG, or PBS at ten day intervals. The neutralizing antibodies and cell-mediated immune responses elicited by the DNA vaccine were detected using serum neutralization tests (SNTs) and ELISAs. At 20 days old, the ducks were challenged with 103EID50 doses of TMUV SD/02 strain and observed for 15 days post challenge. After the second DNA vaccination and during the monitoring period, the levels of TMUV neutralizing antibodies increased in the pVAX1-prM/E-CpG vaccinated ducks. Vaccination with pVAX1-prM/E-CpG resulted in 100.0% protection of the ducks, whereas approximately 40% of ducks vaccinated with pVAX-CpG or PBS manifested clinical symptoms. Expressions of IFN-γ and IL-6 in the pVAX1-prM/E-CpG group were significantly increased (p < 0.01) compared with the control groups during the entire experimental period. The results revealed that a vaccine co-expressing prM and E, and using a CpG-ODN motif as an adjuvant, could elicit effective neutralizing antibody titers and provide efficient protection to ducks against TMUV infection." @default.
• W2976397575 created "2019-10-03" @default.
• W2976397575 creator A5012263451 @default.
• W2976397575 creator A5022499603 @default.
• W2976397575 creator A5050842089 @default.
• W2976397575 creator A5087780600 @default.
• W2976397575 date "2019-12-01" @default.
• W2976397575 modified "2023-10-06" @default.
• W2976397575 title "Evaluation of immunogenicity and protective efficacy of a CpG-adjuvanted DNA vaccine against Tembusu virus" @default.
• W2976397575 cites W1490516138 @default.
• W2976397575 cites W1629663082 @default.
• W2976397575 cites W1930368175 @default.
• W2976397575 cites W1963731038 @default.
• W2976397575 cites W1976943200 @default.
• W2976397575 cites W1989302754 @default.
• W2976397575 cites W2027353448 @default.
• W2976397575 cites W2030046609 @default.
• W2976397575 cites W2032730860 @default.
• W2976397575 cites W2033094913 @default.
• W2976397575 cites W2039642685 @default.
• W2976397575 cites W2046147584 @default.
• W2976397575 cites W2050664835 @default.
• W2976397575 cites W2051298245 @default.
• W2976397575 cites W2058340460 @default.
• W2976397575 cites W2069367707 @default.
• W2976397575 cites W2073742945 @default.
• W2976397575 cites W2084883554 @default.
• W2976397575 cites W2086956767 @default.
• W2976397575 cites W2108244474 @default.
• W2976397575 cites W2110487156 @default.
• W2976397575 cites W2113750146 @default.
• W2976397575 cites W2122180741 @default.
• W2976397575 cites W2127908895 @default.
• W2976397575 cites W2136560021 @default.
• W2976397575 cites W2165645608 @default.
• W2976397575 cites W2177860979 @default.
• W2976397575 cites W2178443088 @default.
• W2976397575 cites W4312607651 @default.
• W2976397575 doi "https://doi.org/10.1016/j.vetimm.2019.109953" @default.
• W2976397575 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31590073" @default.
• W2976397575 hasPublicationYear "2019" @default.
• W2976397575 type Work @default.
• W2976397575 sameAs 2976397575 @default.
• W2976397575 citedByCount "13" @default.
• W2976397575 countsByYear W29763975752020 @default.
• W2976397575 countsByYear W29763975752021 @default.
• W2976397575 countsByYear W29763975752022 @default.
• W2976397575 countsByYear W29763975752023 @default.
• W2976397575 crossrefType "journal-article" @default.
• W2976397575 hasAuthorship W2976397575A5012263451 @default.
• W2976397575 hasAuthorship W2976397575A5022499603 @default.
• W2976397575 hasAuthorship W2976397575A5050842089 @default.
• W2976397575 hasAuthorship W2976397575A5087780600 @default.
• W2976397575 hasConcept C104317684 @default.
• W2976397575 hasConcept C140173407 @default.
• W2976397575 hasConcept C150194340 @default.
• W2976397575 hasConcept C159047783 @default.
• W2976397575 hasConcept C159654299 @default.
• W2976397575 hasConcept C167508917 @default.
• W2976397575 hasConcept C190727270 @default.
• W2976397575 hasConcept C203014093 @default.
• W2976397575 hasConcept C22070199 @default.
• W2976397575 hasConcept C22801619 @default.
• W2976397575 hasConcept C2522874641 @default.
• W2976397575 hasConcept C2777863537 @default.
• W2976397575 hasConcept C2779261636 @default.
• W2976397575 hasConcept C2780801004 @default.
• W2976397575 hasConcept C2780868878 @default.
• W2976397575 hasConcept C40767141 @default.
• W2976397575 hasConcept C55493867 @default.
• W2976397575 hasConcept C86803240 @default.
• W2976397575 hasConcept C8891405 @default.
• W2976397575 hasConceptScore W2976397575C104317684 @default.
• W2976397575 hasConceptScore W2976397575C140173407 @default.
• W2976397575 hasConceptScore W2976397575C150194340 @default.
• W2976397575 hasConceptScore W2976397575C159047783 @default.
• W2976397575 hasConceptScore W2976397575C159654299 @default.
• W2976397575 hasConceptScore W2976397575C167508917 @default.
• W2976397575 hasConceptScore W2976397575C190727270 @default.
• W2976397575 hasConceptScore W2976397575C203014093 @default.
• W2976397575 hasConceptScore W2976397575C22070199 @default.
• W2976397575 hasConceptScore W2976397575C22801619 @default.
• W2976397575 hasConceptScore W2976397575C2522874641 @default.
• W2976397575 hasConceptScore W2976397575C2777863537 @default.
• W2976397575 hasConceptScore W2976397575C2779261636 @default.
• W2976397575 hasConceptScore W2976397575C2780801004 @default.
• W2976397575 hasConceptScore W2976397575C2780868878 @default.
• W2976397575 hasConceptScore W2976397575C40767141 @default.
• W2976397575 hasConceptScore W2976397575C55493867 @default.
• W2976397575 hasConceptScore W2976397575C86803240 @default.
• W2976397575 hasConceptScore W2976397575C8891405 @default.
• W2976397575 hasFunder F4320321001 @default.
• W2976397575 hasLocation W29763975751 @default.
• W2976397575 hasLocation W29763975752 @default.
• W2976397575 hasOpenAccess W2976397575 @default.
• W2976397575 hasPrimaryLocation W29763975751 @default.
• W2976397575 hasRelatedWork W1024660077 @default.
• W2976397575 hasRelatedWork W2055156886 @default.

• next