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- W2976665996 abstract "Abstract Web spiders connect silk proteins, so-called spidroins, into fibers of extraordinary toughness. The spidroin N-terminal domain (NTD) plays a pivotal role in this process: it polymerizes spidroins through a complex mechanism of dimerization. Here we analyze sequences of spidroin NTDs and find an unusually high content of the amino acid methionine. We simultaneously mutate all methionines present in the hydrophobic core of a spidroin NTD from a nursery web spider’s dragline silk to leucine. The mutated NTD is strongly stabilized and folds at the theoretical speed limit. The structure of the mutant is preserved, yet its ability to dimerize is substantially impaired. We find that side chains of core methionines serve to mobilize the fold, which can thereby access various conformations and adapt the association interface for tight binding. Methionine in a hydrophobic core equips a protein with the capacity to dynamically change shape and thus to optimize its function." @default.
- W2976665996 created "2019-10-03" @default.
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- W2976665996 date "2019-09-26" @default.
- W2976665996 modified "2023-10-01" @default.
- W2976665996 title "Methionine in a protein hydrophobic core drives tight interactions required for assembly of spider silk" @default.
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- W2976665996 doi "https://doi.org/10.1038/s41467-019-12365-5" @default.
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