Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977149733> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2977149733 endingPage "929" @default.
- W2977149733 startingPage "924" @default.
- W2977149733 abstract "To study the effect of low-concentration paclitaxel (PTX) on transforming growth factor-β1 (TGF-β1)-induced collagen deposition outside rat pulmonary artery smooth muscle cells (PASMCs) and related mechanism.Primary rat PASMCs were divided into a blank control group (n=3), a model group (n=3), and a drug intervention group (n=3). No treatment was given for the blank control group. The model group was treated with TGF-β1 with a final concentration of 10 ng/mL. The drug intervention group was treated with PTX with a final concentration of 100 nmol/L in addition to the treatment in the model group. MTT colorimetry was used to measure cell proliferation. Quantitative real-time PCR was used to measure the relative mRNA expression of collagen type I (COL-I) and collagen type III (COL-III). ELISA was used to measure the OD value of COL-I and COL-III proteins. Western blot was used to measure the relative protein expression of COL-I, COL-III, and the key proteins of the TGF-β1/Smad3 signaling pathway (Smad3 and p-Smad3).Compared with the blank control group, the model group had significant increases in proliferation ability, relative mRNA and protein expression of COL-I and COL-III, and relative protein expression of p-Smad3 (P<0.05). Compared with the model group, the drug intervention group had significant reductions in the above indicators, but which were still higher than those in the blank control group (P<0.05). There was no significant difference in the relative protein expression of Smad3 among the three groups (P>0.05).Low-concentration PTX exerts a marked inhibitory effect on TGF-β1-induced collagen deposition outside PASMCs, possibly by regulating the phosphorylation of Smad3 protein." @default.
- W2977149733 created "2019-10-03" @default.
- W2977149733 creator A5022646640 @default.
- W2977149733 creator A5034395433 @default.
- W2977149733 creator A5048826252 @default.
- W2977149733 creator A5081178435 @default.
- W2977149733 date "2019-09-01" @default.
- W2977149733 modified "2023-09-23" @default.
- W2977149733 title "[Effect of low-concentration paclitaxel on collagen deposition outside rat pulmonary artery smooth muscle cells and related mechanism]." @default.
- W2977149733 cites W2052399749 @default.
- W2977149733 cites W2065659252 @default.
- W2977149733 cites W2066475375 @default.
- W2977149733 cites W2087307986 @default.
- W2977149733 cites W2118338447 @default.
- W2977149733 cites W2131936721 @default.
- W2977149733 cites W2158748935 @default.
- W2977149733 cites W2265477410 @default.
- W2977149733 cites W2398798493 @default.
- W2977149733 cites W2547353025 @default.
- W2977149733 cites W2612502936 @default.
- W2977149733 cites W2626607827 @default.
- W2977149733 cites W2842768199 @default.
- W2977149733 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/7390237" @default.
- W2977149733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31506155" @default.
- W2977149733 hasPublicationYear "2019" @default.
- W2977149733 type Work @default.
- W2977149733 sameAs 2977149733 @default.
- W2977149733 citedByCount "0" @default.
- W2977149733 crossrefType "journal-article" @default.
- W2977149733 hasAuthorship W2977149733A5022646640 @default.
- W2977149733 hasAuthorship W2977149733A5034395433 @default.
- W2977149733 hasAuthorship W2977149733A5048826252 @default.
- W2977149733 hasAuthorship W2977149733A5081178435 @default.
- W2977149733 hasConcept C104317684 @default.
- W2977149733 hasConcept C105580179 @default.
- W2977149733 hasConcept C118131993 @default.
- W2977149733 hasConcept C126322002 @default.
- W2977149733 hasConcept C134018914 @default.
- W2977149733 hasConcept C153911025 @default.
- W2977149733 hasConcept C185592680 @default.
- W2977149733 hasConcept C2776415932 @default.
- W2977149733 hasConcept C2776694085 @default.
- W2977149733 hasConcept C2777292972 @default.
- W2977149733 hasConcept C2780940725 @default.
- W2977149733 hasConcept C55493867 @default.
- W2977149733 hasConcept C71924100 @default.
- W2977149733 hasConcept C86803240 @default.
- W2977149733 hasConceptScore W2977149733C104317684 @default.
- W2977149733 hasConceptScore W2977149733C105580179 @default.
- W2977149733 hasConceptScore W2977149733C118131993 @default.
- W2977149733 hasConceptScore W2977149733C126322002 @default.
- W2977149733 hasConceptScore W2977149733C134018914 @default.
- W2977149733 hasConceptScore W2977149733C153911025 @default.
- W2977149733 hasConceptScore W2977149733C185592680 @default.
- W2977149733 hasConceptScore W2977149733C2776415932 @default.
- W2977149733 hasConceptScore W2977149733C2776694085 @default.
- W2977149733 hasConceptScore W2977149733C2777292972 @default.
- W2977149733 hasConceptScore W2977149733C2780940725 @default.
- W2977149733 hasConceptScore W2977149733C55493867 @default.
- W2977149733 hasConceptScore W2977149733C71924100 @default.
- W2977149733 hasConceptScore W2977149733C86803240 @default.
- W2977149733 hasIssue "9" @default.
- W2977149733 hasLocation W29771497331 @default.
- W2977149733 hasOpenAccess W2977149733 @default.
- W2977149733 hasPrimaryLocation W29771497331 @default.
- W2977149733 hasRelatedWork W1980194808 @default.
- W2977149733 hasRelatedWork W1989634017 @default.
- W2977149733 hasRelatedWork W2000907318 @default.
- W2977149733 hasRelatedWork W2004173419 @default.
- W2977149733 hasRelatedWork W2035810202 @default.
- W2977149733 hasRelatedWork W2049047086 @default.
- W2977149733 hasRelatedWork W2127549094 @default.
- W2977149733 hasRelatedWork W2368391411 @default.
- W2977149733 hasRelatedWork W2392371029 @default.
- W2977149733 hasRelatedWork W4255947271 @default.
- W2977149733 hasVolume "21" @default.
- W2977149733 isParatext "false" @default.
- W2977149733 isRetracted "false" @default.
- W2977149733 magId "2977149733" @default.
- W2977149733 workType "article" @default.