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- W2977249987 abstract "Objective: To clarify the expression and regulation mechanism of the new target gene (HCTP4) transactivat-ed by core protein of hepatitis C virus (HCV) .Methods: Four hundred and forty-one base pairs (bp) upstream of the translation start site was selected as promoter sequence which was amplified from hepatoblastoma cell line-HepG2 cell genomic DNA by polymerase chain reaction (PCR) .The amplified product was cloned into pCAT3 vector. The HepG2 cell line was tranfect-ed by pCAT3-HCTP4-promoter.The CAT activity was detected by an enzyme-linked immunoassay (ELISA) kit.Results: We found pCAT3-HCTP4-promoter had activity of CAT. The expression of CAT was 5 times as higher as pCAT3-basic control vector. Conclusion: These results indicated that the pCAT3-HCTP4-promoter has promoter activity." @default.
- W2977249987 created "2019-10-10" @default.
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- W2977249987 date "2003-01-01" @default.
- W2977249987 modified "2023-09-24" @default.
- W2977249987 title "Identification and Evaluation Promoter Sequence and Its Transcription Activation of the HCTP4 Transactivated by Core Protein of Hepatitis C Virus" @default.
- W2977249987 hasPublicationYear "2003" @default.
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