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- W2977258702 abstract "It was compared that the effects of different sampling time,the methods of pretreatments,dissociations time and dyeing time on chromosome preparation of tree Lonicera japonica shoot tips.The results showed that the best experimental protocol for karyotype analysis of tree Lonicera japonica.is better when sampling time is between 10:00AM and 11:00AM,the pretreatment time with saturated p-dichlorobenzene lasts 3-4h,the dissociation with 1mol/L HCl is at 60℃ for 13min,and the dyeing time lasts 10min with modified carbolic acid-magenta.Simultaneously,the karyotype analysis also showed that the karyotype formulae of tree Lonicera japonica is 2n=18= 8m +8sm+2st,where one satellite was found in the ninth chromosomes,the relative length of the chromosome composition was 2n=18=4L+8M2+4M1+2S,belonging to 2B type.Its index of Asymmetric was 64.47%,and the karyotype asymmetry belongs to transition type from symmetry type to asymmetry one,suggesting that tree Lonicera japonica is relatively evolutionary form compared to wild type Lonicera japonica." @default.
- W2977258702 created "2019-10-10" @default.
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- W2977258702 date "2009-01-01" @default.
- W2977258702 modified "2023-09-25" @default.
- W2977258702 title "Optimization of Chromosome Mounting Technique and Karyotype Analysis of Tree Lonicera japonica" @default.
- W2977258702 hasPublicationYear "2009" @default.
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