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- W2977325101 abstract "Objectives To establish the method of RHD genotyping by PCR SSP and preliminary study on RHD gene polymorphism among apparently RhD negative Chinese.Methods The polymerase chain reaction with sequence specific primers (PCR SSP) based RhD genotyping method has been developed using eight RHD specific primers designed to amplify RHD exon 3, 4, 5, 6, 7, 9, 10 and intron 4.Using the PCR SSP method,the RHD gene polymorphism was studied among 115 cases of apparently RhD negative Chinese who were identified by the routine serological methods and absorption elution tests.Results Among 115 RhD negative Chinese,88 samples (76.5%) were confirmed as RhD negative and 27 samples (23.5%) were Del phenotype by the absorption elution tests.Using PCR SSP method,RHD intron 4 were detected in all Del individuals.17 individuals from the 115 cases were studied for seven exons of RHD,6 Del cases were found to carry intact RHD gene.11 cases that confirmed as RhD negative showed 7 cases with total deletion of RHD gene,2 cases with intact RHD gene,1 case with the deletion of RHD exon 5 and 1 case carrying only RHD exon 10.Conclusions The PCR SSP could be used to study RHD genotype.Del phenotypes were highly detected among the apparently RhD negative Chinese individuals and Del cases had the possibility of carrying the intact RHD gene.The apparently RhD negative Chinese individuals identified also had the possibility of carrying the RHD gene and showed the RHD gene polymorphism.The RhD negative Chinese carried RHD gene were found the RHc phenotype which was different from the reported all RhC phenotypes among the RhD negative Chinese." @default.
- W2977325101 created "2019-10-10" @default.
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- W2977325101 date "2003-01-01" @default.
- W2977325101 modified "2023-09-28" @default.
- W2977325101 title "Study on RHD gene polymorphism among the apparently RhD-negative Chinese" @default.
- W2977325101 hasPublicationYear "2003" @default.
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