FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977564293> ?p ?o ?g. }

• W2977564293 endingPage "126" @default.
• W2977564293 startingPage "115" @default.
• W2977564293 abstract "Stem cells have essential applications in in vitro tissue engineering or regenerative medicine. However, there is still a need to understand more deeply the mechanisms of stem cell differentiation and to optimize the methods to control stem cell function. In this study, we first investigated the activity of DNA methyltransferases (DNMTs) during chondrogenic differentiation of human bone marrow-derived mesenchymal stem/progenitor cells (hBMSCs) and found that DNMT3A and DNMT3B were markedly upregulated during hBMSC chondrogenic differentiation. In an attempt to understand the effect of DNMT3A and DNMT3B on the chondrogenic differentiation of hBMSCs, we transiently transfected the cells with expression vectors for the two enzymes. Interestingly, DNMT3A overexpression strongly enhanced the chondrogenesis of hBMSCs, by increasing the gene expression of the mature chondrocyte marker, collagen type II, more than 200-fold. Analysis of the methylation condition in the cells revealed that DNMT3A and DNMT3B methylated the promoter sequence of early stem cell markers, <i>NANOG</i> and <i>POU5F1</i>(OCT-4). Conversely, the suppression of chondrogenic differentiation and the increase in stem cell markers of hBMSCs were obtained by chemical stimulation with the demethylating agent, 5-azacitidine. Loss-of-function assays with siRNAs targeting DNMT3A also significantly suppressed the chondrogenic differentiation of hBMSCs. Together, these results not only show the critical roles of DNMTs in regulating the chondrogenic differentiation of hBMSCs, but also suggest that manipulation of DNMT activity can be important tools to enhance the differentiation of hBMSCs towards chondrogenesis for potential application in cartilage tissue engineering or cartilage regeneration." @default.
• W2977564293 created "2019-10-10" @default.
• W2977564293 creator A5030330429 @default.
• W2977564293 creator A5034652400 @default.
• W2977564293 creator A5035336989 @default.
• W2977564293 creator A5046053082 @default.
• W2977564293 creator A5056787767 @default.
• W2977564293 creator A5060349255 @default.
• W2977564293 creator A5073754156 @default.
• W2977564293 creator A5076149190 @default.
• W2977564293 creator A5076318169 @default.
• W2977564293 creator A5088239745 @default.
• W2977564293 date "2019-01-01" @default.
• W2977564293 modified "2023-09-27" @default.
• W2977564293 title "DNA Methylation-Based Regulation of Human Bone Marrow-Derived Mesenchymal Stem/Progenitor Cell Chondrogenic Differentiation" @default.
• W2977564293 cites W1608008717 @default.
• W2977564293 cites W1923389664 @default.
• W2977564293 cites W1970752925 @default.
• W2977564293 cites W1996949698 @default.
• W2977564293 cites W2024805424 @default.
• W2977564293 cites W2041191257 @default.
• W2977564293 cites W2042473783 @default.
• W2977564293 cites W2046241907 @default.
• W2977564293 cites W2047418655 @default.
• W2977564293 cites W2061946349 @default.
• W2977564293 cites W2065786046 @default.
• W2977564293 cites W2074068088 @default.
• W2977564293 cites W2081264270 @default.
• W2977564293 cites W2084118479 @default.
• W2977564293 cites W2091203167 @default.
• W2977564293 cites W2108164787 @default.
• W2977564293 cites W2108241558 @default.
• W2977564293 cites W2119103258 @default.
• W2977564293 cites W2124049725 @default.
• W2977564293 cites W2126263466 @default.
• W2977564293 cites W2142801671 @default.
• W2977564293 cites W2144873726 @default.
• W2977564293 cites W2154150104 @default.
• W2977564293 cites W2163930755 @default.
• W2977564293 cites W2169079272 @default.
• W2977564293 cites W2267868386 @default.
• W2977564293 cites W2338309018 @default.
• W2977564293 cites W2341664663 @default.
• W2977564293 cites W2409356434 @default.
• W2977564293 cites W2586232176 @default.
• W2977564293 cites W2887357191 @default.
• W2977564293 cites W602773616 @default.
• W2977564293 doi "https://doi.org/10.1159/000502885" @default.
• W2977564293 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31574516" @default.
• W2977564293 hasPublicationYear "2019" @default.
• W2977564293 type Work @default.
• W2977564293 sameAs 2977564293 @default.
• W2977564293 citedByCount "8" @default.
• W2977564293 countsByYear W29775642932020 @default.
• W2977564293 countsByYear W29775642932021 @default.
• W2977564293 countsByYear W29775642932022 @default.
• W2977564293 countsByYear W29775642932023 @default.
• W2977564293 crossrefType "journal-article" @default.
• W2977564293 hasAuthorship W2977564293A5030330429 @default.
• W2977564293 hasAuthorship W2977564293A5034652400 @default.
• W2977564293 hasAuthorship W2977564293A5035336989 @default.
• W2977564293 hasAuthorship W2977564293A5046053082 @default.
• W2977564293 hasAuthorship W2977564293A5056787767 @default.
• W2977564293 hasAuthorship W2977564293A5060349255 @default.
• W2977564293 hasAuthorship W2977564293A5073754156 @default.
• W2977564293 hasAuthorship W2977564293A5076149190 @default.
• W2977564293 hasAuthorship W2977564293A5076318169 @default.
• W2977564293 hasAuthorship W2977564293A5088239745 @default.
• W2977564293 hasBestOaLocation W29775642932 @default.
• W2977564293 hasConcept C104317684 @default.
• W2977564293 hasConcept C148738053 @default.
• W2977564293 hasConcept C150194340 @default.
• W2977564293 hasConcept C185592680 @default.
• W2977564293 hasConcept C190727270 @default.
• W2977564293 hasConcept C198826908 @default.
• W2977564293 hasConcept C201750760 @default.
• W2977564293 hasConcept C28328180 @default.
• W2977564293 hasConcept C35496256 @default.
• W2977564293 hasConcept C54355233 @default.
• W2977564293 hasConcept C86803240 @default.
• W2977564293 hasConcept C95444343 @default.
• W2977564293 hasConceptScore W2977564293C104317684 @default.
• W2977564293 hasConceptScore W2977564293C148738053 @default.
• W2977564293 hasConceptScore W2977564293C150194340 @default.
• W2977564293 hasConceptScore W2977564293C185592680 @default.
• W2977564293 hasConceptScore W2977564293C190727270 @default.
• W2977564293 hasConceptScore W2977564293C198826908 @default.
• W2977564293 hasConceptScore W2977564293C201750760 @default.
• W2977564293 hasConceptScore W2977564293C28328180 @default.
• W2977564293 hasConceptScore W2977564293C35496256 @default.
• W2977564293 hasConceptScore W2977564293C54355233 @default.
• W2977564293 hasConceptScore W2977564293C86803240 @default.
• W2977564293 hasConceptScore W2977564293C95444343 @default.
• W2977564293 hasIssue "3-4" @default.
• W2977564293 hasLocation W29775642931 @default.
• W2977564293 hasLocation W29775642932 @default.
• W2977564293 hasLocation W29775642933 @default.
• W2977564293 hasLocation W29775642934 @default.

• next