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- W2977637515 abstract "Aim To connect the full cDNA of hSGLT1 and hSGLT2 with the vector pReceiver-M03 and transfect them to CHO-K1 cells,and to investigate the effects of mangiferin on hSGLT1 and hSGLT2 expression in CHO-K1-SGLT1 and CHO-K1-SGLT2,compared to phloridzin as a positive control.Methods The full length cDNA of SGLT1 and SGLT2 was connected to the vector pReceiver-M03 and transfected into CHO-K1 cells by Lipofectamine2000.The expression of exogenous genes was identified by fluorescence microscope.The stable transfect cells would be obtained by screening of G418.The changes of SGLT1 and SGLT2 expression in CHO-K1-SGLT1 and CHO-K1-SGLT2 exposure to mangiferin or phloridzin were observed by the Western blot.Results Forty-eight hours after transfection,the circular green fluorescence in the cell membrane was observed under fluorescence microscope.The transfection efficiency was 60%~70%.The results of Western blot suggested that hSGLT1 and hSGLT2 expressed in CHO-K1 cells.Moreover,48 hours after CHO-K1-SGLT1 and CHO-K1-SGLT2 exposure to mangiferin or phloridzin,Western blot analysis revealed that hSGLT1/β-actin and hSGLT2/β-actin were significantly decreased compared with untreated cells(P0.05),but the effect of mangiferin was not significant compared to phloridzin group(P0.05).Conclusions The stable transfected cells have been obtained successfully.Mangiferin can inhibit the expression of SGLT1 and SGLT2,which is similar to the effect of phloridzin." @default.
- W2977637515 created "2019-10-10" @default.
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- W2977637515 date "2013-01-01" @default.
- W2977637515 modified "2023-09-28" @default.
- W2977637515 title "Establishment of CHO-K1 cell lines stably expressing hSGLTs structure protein and effect of mangiferin on expression of SGLTs" @default.
- W2977637515 hasPublicationYear "2013" @default.
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