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- W2977729042 abstract "In this study, the random amplified polymorphic DNA(RAPD) technique was used to analyze the genetic variation among the allotetraploid hybrid of red crucian carp (Carassius auratus red var.,♀) × common carp(Cyprinus carpio L.♂), red crucian carp and common carp. One hundred 10-nucleotide-long random primers were used in the RAPD analysis. Of these primers, 60 primers produced well-amplified and reproducible band patterns. There were 6 to 20 bands ranging in size from about 200 to 3 500 bp in the amplified products of a single primer. After excluding bands that were not clearly identifiable, a total of 1 554 distinguishable fragments were compiled for phylogenetic relationship analysis. The genetic similarity index of the amplified DNA bands between the allotetraploid hybrid and their original maternal red crucian carp was 69.41 %, and the genetic similarity index of the amplified DNA bands between allotetraploid hybrid and their original paternal common carp was 64.47 %. The similarity index of the amplified DNA bands between red crucian carp and common carp was 42.18 % . Moreover , 4 specific bands were found only in the allotetraploid hybrid, the non-parentage bands ratio of the allotetraploid hybrid was 0.72 % . The results not only provided the specific molecular markers of the allotetraploid hybrid , but also demonstrated that the allotetraploid hybrid inherited a little more genetic materials from their original maternal red crucian carp than their original paternal common carp." @default.
- W2977729042 created "2019-10-10" @default.
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- W2977729042 date "2003-01-01" @default.
- W2977729042 modified "2023-09-27" @default.
- W2977729042 title "RAPD analysis of genetic variation between the allotetraploid hybrid of red crucian carp × common carp and their original parents" @default.
- W2977729042 hasPublicationYear "2003" @default.
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