FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977771709> ?p ?o ?g. }

• W2977771709 endingPage "20190081" @default.
• W2977771709 startingPage "20190081" @default.
• W2977771709 abstract "The mechanical performance of cardiomyocytes (CMs) is an important indicator of their maturation state and of primary importance for the development of therapies based on cardiac stem cells. As the mechanical analysis of adherent cells at high-throughput remains challenging, we explore the applicability of real-time deformability cytometry (RT-DC) to probe cardiomyocytes in suspension. RT-DC is a microfluidic technology allowing for real-time mechanical analysis of thousands of cells with a throughput exceeding 1000 cells per second. For CMs derived from human-induced pluripotent stem cells, we determined a Young's modulus of 1.25 ± 0.08 kPa which is in close range to previous reports. Upon challenging the cytoskeleton with cytochalasin D (CytoD) to induce filamentous actin depolymerization, we distinguish three different regimes in cellular elasticity. Transitions are observed below 10 nM and above 10 3 nM and are characterized by a decrease in Young's modulus. These regimes can be linked to cytoskeletal and sarcomeric actin contributions by CM contractility measurements at varying CytoD concentrations, where we observe a significant reduction in pulse duration only above 10 3 nM while no change is found for compound exposure at lower concentrations. Comparing our results to mechanical cell measurements using atomic force microscopy, we demonstrate for the first time to our knowledge, the feasibility of using a microfluidic technique to measure mechanical properties of large samples of adherent cells while linking our results to the composition of the cytoskeletal network. This article is part of a discussion meeting issue ‘Single cell ecology'." @default.
• W2977771709 created "2019-10-10" @default.
• W2977771709 creator A5019367873 @default.
• W2977771709 creator A5046973722 @default.
• W2977771709 creator A5049341595 @default.
• W2977771709 creator A5061641950 @default.
• W2977771709 creator A5071359939 @default.
• W2977771709 date "2019-10-07" @default.
• W2977771709 modified "2023-10-12" @default.
• W2977771709 title "Cardiomyocyte mechanodynamics under conditions of actin remodelling" @default.
• W2977771709 cites W1730627877 @default.
• W2977771709 cites W1747672433 @default.
• W2977771709 cites W1970526294 @default.
• W2977771709 cites W1991191399 @default.
• W2977771709 cites W1994109845 @default.
• W2977771709 cites W2002221267 @default.
• W2977771709 cites W2016983464 @default.
• W2977771709 cites W2034713721 @default.
• W2977771709 cites W2037180748 @default.
• W2977771709 cites W2046178807 @default.
• W2977771709 cites W2053677311 @default.
• W2977771709 cites W2059495870 @default.
• W2977771709 cites W2060167445 @default.
• W2977771709 cites W2061680513 @default.
• W2977771709 cites W2068459121 @default.
• W2977771709 cites W2079986178 @default.
• W2977771709 cites W2080544569 @default.
• W2977771709 cites W2086557128 @default.
• W2977771709 cites W2090588873 @default.
• W2977771709 cites W2098389304 @default.
• W2977771709 cites W2106562564 @default.
• W2977771709 cites W2120616941 @default.
• W2977771709 cites W2129314813 @default.
• W2977771709 cites W2133214533 @default.
• W2977771709 cites W2138033616 @default.
• W2977771709 cites W2140802783 @default.
• W2977771709 cites W2141118238 @default.
• W2977771709 cites W2144517964 @default.
• W2977771709 cites W2163802608 @default.
• W2977771709 cites W2173240866 @default.
• W2977771709 cites W2175892199 @default.
• W2977771709 cites W2200325631 @default.
• W2977771709 cites W2214197409 @default.
• W2977771709 cites W2265505631 @default.
• W2977771709 cites W2329686860 @default.
• W2977771709 cites W2462897858 @default.
• W2977771709 cites W2574430960 @default.
• W2977771709 cites W2596201013 @default.
• W2977771709 cites W2608402435 @default.
• W2977771709 cites W2611659007 @default.
• W2977771709 cites W2618457026 @default.
• W2977771709 cites W2619325259 @default.
• W2977771709 cites W2621098621 @default.
• W2977771709 cites W274696614 @default.
• W2977771709 cites W2762399116 @default.
• W2977771709 cites W2764157960 @default.
• W2977771709 cites W2766689274 @default.
• W2977771709 cites W2783266799 @default.
• W2977771709 cites W2796144999 @default.
• W2977771709 cites W2805295001 @default.
• W2977771709 cites W2805703925 @default.
• W2977771709 cites W2809787003 @default.
• W2977771709 cites W2883029425 @default.
• W2977771709 cites W2901217778 @default.
• W2977771709 cites W2904261171 @default.
• W2977771709 cites W2912915283 @default.
• W2977771709 cites W2995968923 @default.
• W2977771709 doi "https://doi.org/10.1098/rstb.2019.0081" @default.
• W2977771709 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6792454" @default.
• W2977771709 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31587648" @default.
• W2977771709 hasPublicationYear "2019" @default.
• W2977771709 type Work @default.
• W2977771709 sameAs 2977771709 @default.
• W2977771709 citedByCount "17" @default.
• W2977771709 countsByYear W29777717092019 @default.
• W2977771709 countsByYear W29777717092020 @default.
• W2977771709 countsByYear W29777717092021 @default.
• W2977771709 countsByYear W29777717092022 @default.
• W2977771709 countsByYear W29777717092023 @default.
• W2977771709 crossrefType "journal-article" @default.
• W2977771709 hasAuthorship W2977771709A5019367873 @default.
• W2977771709 hasAuthorship W2977771709A5046973722 @default.
• W2977771709 hasAuthorship W2977771709A5049341595 @default.
• W2977771709 hasAuthorship W2977771709A5061641950 @default.
• W2977771709 hasAuthorship W2977771709A5071359939 @default.
• W2977771709 hasBestOaLocation W29777717091 @default.
• W2977771709 hasConcept C12554922 @default.
• W2977771709 hasConcept C125705527 @default.
• W2977771709 hasConcept C134018914 @default.
• W2977771709 hasConcept C142669718 @default.
• W2977771709 hasConcept C1491633281 @default.
• W2977771709 hasConcept C171250308 @default.
• W2977771709 hasConcept C185592680 @default.
• W2977771709 hasConcept C192562407 @default.
• W2977771709 hasConcept C2775995422 @default.
• W2977771709 hasConcept C2776041460 @default.
• W2977771709 hasConcept C2780171871 @default.
• W2977771709 hasConcept C2780339063 @default.

• next