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- W297777744 abstract "The consortium constructed a bacterial artificial chromosome (BAC) map and described its integration with previous landmark maps of the human genome. Clones corresponding to specific STS markers were screened with genomic BAC and PAC libraries by PCR or by probe hybridization using overgo probes. Fingerprints were generated from marker-positive clones using HindIII restriction enzyme digests. Probes were used to generate the HindIII fingerprints using the Prime-it Fluor labeling kit, and hybridized to chromosome spreads on slides with competitor DNA present. The sequenced clones were integrated using synthetic fingerprints from in silico digests. Based on the map of the whole genome to enable the selection of clones for sequencing and for the accurate assembly of the genome sequence, the consortium constructed the whole-genome bacterial artificial chromosome map and integrated it with previous landmark maps. Information from mapping efforts focused on specific regions. The consortium also obtained the integration of sequence data with the map. The clone-based map has been vital for the accurate assembly of the human genome sequence. The whole-genome bacterial artificial chromosome clones comprising the clone-based map also provides an integrated resource for analysis of chromosome structure, comparative genome hybridization, and functional genetics, including gene inactivation. Together, the human genome clone map and the anchored sequence map provide synergistic resources for future analysis of the human genome.—Hans E. Grossniklaus" @default.
- W297777744 created "2016-06-24" @default.
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- W297777744 date "2001-08-01" @default.
- W297777744 modified "2023-09-25" @default.
- W297777744 title "A physical map of the human genome. The International Human Genome Mapping Consortium. Nature 2001;409:934–941." @default.
- W297777744 doi "https://doi.org/10.1016/s0002-9394(01)01072-8" @default.
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