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- W2977814193 abstract "Objective The attachment and penetration of in vitro cultured human gingival fibroblasts on degradable membrane scaffords were studied in order to fabricate a tissue engineering artificial gingival for repairing gingival defects and atrophy.Methods Human gingival fibroblasts were isolated from healthy human gingiva around the teeth of 5 patients, and cultured with DMEM. The fourth generation of cultured gingival fibrablasts were seeded onto 2 types of degradable scaffolds, in a giving density, then the cell numbers were assayed with LDH kit. The number of penetrated cell through membrane was measured under microscope and the penetration of cell was calculated. Results The attachment and penetration role of human gingival fibroblasts on GC scafford was higher than that on Gore-resolut XT scafford with statistical significant difference. Conclusion The GC membrane scafford pocesses good biocompatibility and cell affinity, its pore diameter and degrading rate are helpful for human gingival fibroblasts attachment and growth. It is appropriate to act as an in vivo tissue engineering scafford for artificial gingival." @default.
- W2977814193 created "2019-10-10" @default.
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- W2977814193 date "2002-01-01" @default.
- W2977814193 modified "2023-09-25" @default.
- W2977814193 title "Study on the Attachment and Spreading of Cultured Human Gingival Fibroblasts upon Degradable Scaffolds" @default.
- W2977814193 hasPublicationYear "2002" @default.
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