FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977856874> ?p ?o ?g. }

• W2977856874 endingPage "351" @default.
• W2977856874 startingPage "338" @default.
• W2977856874 abstract "Bioengineering immune cells via gene therapy offers treatment opportunities for currently fatal viral infections. Also cell therapeutics offer most recently a breakthrough technology to combat cancer. These primary human cells, however, are sensitive to toxic influences, which make the utilization of optimized physical transfection techniques necessary. The otherwise commonly applied delivery agents such as LipofectamineⓇ or strongly cationic polymer structures are not only unsuitable for in vivo experiments, but are also highly toxic to immune cells. This study aimed to improve the design of polymeric carrier systems for small interfering RNA, which would allow efficient internalization into CD8+T-cells without affecting their viability and thereby removing the current limitations in the field. Here, two new carrier systems for small interfering RNA were tested. One is a cationic diblock copolymer, in which less than 10% of the monomers were modified with triphenylphosphonium cations. This moiety is lipophilic, promotes uptake and it is mostly known for its mitotropic properties. Furthermore, cationic nanohydrogel particles were synthesized in exceedingly small sizes (Rh < 14 nm). After full physicochemical characterization of the two carriers, extensive cytotoxicity studies were performed and the concentration dependent uptake into CD8+T-cells was tested in correlation to incubation time and protein content of the surrounding medium. Both carriers facilitated efficient complexation of siRNA as well as significant internalization into primary human cells in less than three hours of incubation. In addition, neither of the delivery systems reduced cell viability making them good candidates to transport siRNA into CD8+T-cells efficiently. STATEMENT OF SIGNIFICANCE: This study provides insights into the design of polymeric delivery agents as the method of choice for overcoming the limitations of cell manipulation. Until now, CD8+T-cells, which have become a treatment tool for currently fatal diseases, have not yet been made accessible for gene silencing by polymeric siRNA carrier systems. Choosing appropriate modification approaches for two chemically different polymer structures, we were, in both cases, able to achieve significant uptake in these cells even at low concentrations and without inducing cytotoxicity. These results remove current limitations and pave the way for bioengineering via gene therapy." @default.
• W2977856874 created "2019-10-10" @default.
• W2977856874 creator A5001477363 @default.
• W2977856874 creator A5004185289 @default.
• W2977856874 creator A5011225301 @default.
• W2977856874 creator A5012985500 @default.
• W2977856874 creator A5019031942 @default.
• W2977856874 creator A5021657328 @default.
• W2977856874 creator A5034838011 @default.
• W2977856874 creator A5038088027 @default.
• W2977856874 creator A5053405743 @default.
• W2977856874 creator A5055241475 @default.
• W2977856874 creator A5056387502 @default.
• W2977856874 date "2019-12-01" @default.
• W2977856874 modified "2023-10-01" @default.
• W2977856874 title "Overcoming the barrier of CD8+ T cells: Two types of nano-sized carriers for siRNA transport" @default.
• W2977856874 cites W1481615440 @default.
• W2977856874 cites W16266328 @default.
• W2977856874 cites W1752114428 @default.
• W2977856874 cites W1975045295 @default.
• W2977856874 cites W1976556354 @default.
• W2977856874 cites W1984568212 @default.
• W2977856874 cites W1985469208 @default.
• W2977856874 cites W1988683630 @default.
• W2977856874 cites W1997371191 @default.
• W2977856874 cites W2010300252 @default.
• W2977856874 cites W2010619482 @default.
• W2977856874 cites W2014760202 @default.
• W2977856874 cites W2016705082 @default.
• W2977856874 cites W2021610469 @default.
• W2977856874 cites W2024414783 @default.
• W2977856874 cites W2024840420 @default.
• W2977856874 cites W2028773523 @default.
• W2977856874 cites W2029823051 @default.
• W2977856874 cites W2032576390 @default.
• W2977856874 cites W2032881319 @default.
• W2977856874 cites W2039109946 @default.
• W2977856874 cites W2041452578 @default.
• W2977856874 cites W2043268922 @default.
• W2977856874 cites W2052611341 @default.
• W2977856874 cites W2060179384 @default.
• W2977856874 cites W2069466069 @default.
• W2977856874 cites W2073026167 @default.
• W2977856874 cites W2085497998 @default.
• W2977856874 cites W2093811077 @default.
• W2977856874 cites W2094652659 @default.
• W2977856874 cites W2113797423 @default.
• W2977856874 cites W2114160655 @default.
• W2977856874 cites W2133693793 @default.
• W2977856874 cites W2139004599 @default.
• W2977856874 cites W2145140887 @default.
• W2977856874 cites W2151707711 @default.
• W2977856874 cites W2154663577 @default.
• W2977856874 cites W2157981287 @default.
• W2977856874 cites W2164833177 @default.
• W2977856874 cites W2166721734 @default.
• W2977856874 cites W2277242640 @default.
• W2977856874 cites W2323554009 @default.
• W2977856874 cites W2434909612 @default.
• W2977856874 cites W2559911871 @default.
• W2977856874 cites W2625064767 @default.
• W2977856874 doi "https://doi.org/10.1016/j.actbio.2019.10.006" @default.
• W2977856874 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31586726" @default.
• W2977856874 hasPublicationYear "2019" @default.
• W2977856874 type Work @default.
• W2977856874 sameAs 2977856874 @default.
• W2977856874 citedByCount "10" @default.
• W2977856874 countsByYear W29778568742020 @default.
• W2977856874 countsByYear W29778568742021 @default.
• W2977856874 countsByYear W29778568742022 @default.
• W2977856874 countsByYear W29778568742023 @default.
• W2977856874 crossrefType "journal-article" @default.
• W2977856874 hasAuthorship W2977856874A5001477363 @default.
• W2977856874 hasAuthorship W2977856874A5004185289 @default.
• W2977856874 hasAuthorship W2977856874A5011225301 @default.
• W2977856874 hasAuthorship W2977856874A5012985500 @default.
• W2977856874 hasAuthorship W2977856874A5019031942 @default.
• W2977856874 hasAuthorship W2977856874A5021657328 @default.
• W2977856874 hasAuthorship W2977856874A5034838011 @default.
• W2977856874 hasAuthorship W2977856874A5038088027 @default.
• W2977856874 hasAuthorship W2977856874A5053405743 @default.
• W2977856874 hasAuthorship W2977856874A5055241475 @default.
• W2977856874 hasAuthorship W2977856874A5056387502 @default.
• W2977856874 hasConcept C104317684 @default.
• W2977856874 hasConcept C109316439 @default.
• W2977856874 hasConcept C12554922 @default.
• W2977856874 hasConcept C139770010 @default.
• W2977856874 hasConcept C1491633281 @default.
• W2977856874 hasConcept C171250308 @default.
• W2977856874 hasConcept C185592680 @default.
• W2977856874 hasConcept C192562407 @default.
• W2977856874 hasConcept C202751555 @default.
• W2977856874 hasConcept C203014093 @default.
• W2977856874 hasConcept C22615655 @default.
• W2977856874 hasConcept C40767141 @default.
• W2977856874 hasConcept C49923114 @default.
• W2977856874 hasConcept C53227056 @default.
• W2977856874 hasConcept C54009773 @default.

• next