FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977882356> ?p ?o ?g. }

• W2977882356 abstract "Abstract Background Genome wide association studies (GWAS) are a powerful tool for identifying quantitative trait loci (QTL) and causal single nucleotide polymorphisms (SNPs)/genes associated with various important traits in crop species. Typically, GWAS in crops are performed using a panel of inbred lines, where multiple replicates of the same inbred are measured and the average phenotype is taken as the response variable. Here we describe and evaluate single plant GWAS (sp-GWAS) for performing a GWAS on individual plants, which does not require an association panel of inbreds. Instead sp-GWAS relies on the phenotypes and genotypes from individual plants sampled from a randomly mating population. Importantly, we demonstrate how sp-GWAS can be efficiently combined with a bulk segregant analysis (BSA) experiment to rapidly corroborate evidence for significant SNPs. Results In this study we used the Shoepeg maize landrace, collected as an open pollinating variety from a farm in Southern Missouri in the 1960’s, to evaluate whether sp-GWAS coupled with BSA can efficiently and powerfully used to detect significant association of SNPs for plant height (PH). Plant were grown in 8 locations across two years and in total 768 individuals were genotyped and phenotyped for sp-GWAS. A total of 306 k polymorphic markers in 768 individuals evaluated via association analysis detected 25 significant SNPs ( P ≤ 0.00001) for PH. The results from our single-plant GWAS were further validated by bulk segregant analysis (BSA) for PH. BSA sequencing was performed on the same population by selecting tall and short plants as separate bulks. This approach identified 37 genomic regions for plant height. Of the 25 significant SNPs from GWAS, the three most significant SNPs co-localize with regions identified by BSA. Conclusion Overall, this study demonstrates that sp-GWAS coupled with BSA can be a useful tool for detecting significant SNPs and identifying candidate genes. This result is particularly useful for species/populations where association panels are not readily available." @default.
• W2977882356 created "2019-10-10" @default.
• W2977882356 creator A5013671164 @default.
• W2977882356 creator A5025927794 @default.
• W2977882356 creator A5034383737 @default.
• W2977882356 creator A5061885595 @default.
• W2977882356 creator A5082487928 @default.
• W2977882356 date "2019-10-08" @default.
• W2977882356 modified "2023-10-18" @default.
• W2977882356 title "Single-plant GWAS coupled with bulk segregant analysis allows rapid identification and corroboration of plant-height candidate SNPs" @default.
• W2977882356 cites W1538537184 @default.
• W2977882356 cites W1551468003 @default.
• W2977882356 cites W1598342510 @default.
• W2977882356 cites W1816932740 @default.
• W2977882356 cites W1927774076 @default.
• W2977882356 cites W1966543808 @default.
• W2977882356 cites W1966914636 @default.
• W2977882356 cites W1970311849 @default.
• W2977882356 cites W1972216668 @default.
• W2977882356 cites W1976336296 @default.
• W2977882356 cites W1983544851 @default.
• W2977882356 cites W1988144964 @default.
• W2977882356 cites W1995446034 @default.
• W2977882356 cites W1995517077 @default.
• W2977882356 cites W1998465340 @default.
• W2977882356 cites W1999102617 @default.
• W2977882356 cites W2003865229 @default.
• W2977882356 cites W2005712596 @default.
• W2977882356 cites W2014704298 @default.
• W2977882356 cites W2018835335 @default.
• W2977882356 cites W2019182222 @default.
• W2977882356 cites W2020888950 @default.
• W2977882356 cites W2024278789 @default.
• W2977882356 cites W2025023616 @default.
• W2977882356 cites W2027540844 @default.
• W2977882356 cites W2033151532 @default.
• W2977882356 cites W2033750203 @default.
• W2977882356 cites W2039013541 @default.
• W2977882356 cites W2039405433 @default.
• W2977882356 cites W2042143257 @default.
• W2977882356 cites W2050685506 @default.
• W2977882356 cites W2055933090 @default.
• W2977882356 cites W2061622616 @default.
• W2977882356 cites W2063119385 @default.
• W2977882356 cites W2063368379 @default.
• W2977882356 cites W2065690099 @default.
• W2977882356 cites W2068069733 @default.
• W2977882356 cites W2068235688 @default.
• W2977882356 cites W2068406373 @default.
• W2977882356 cites W2072715465 @default.
• W2977882356 cites W2075663425 @default.
• W2977882356 cites W2080183225 @default.
• W2977882356 cites W2080437745 @default.
• W2977882356 cites W2082380971 @default.
• W2977882356 cites W2082536587 @default.
• W2977882356 cites W2091473030 @default.
• W2977882356 cites W2104905104 @default.
• W2977882356 cites W2106590854 @default.
• W2977882356 cites W2108229331 @default.
• W2977882356 cites W2113280067 @default.
• W2977882356 cites W2113492305 @default.
• W2977882356 cites W2114451730 @default.
• W2977882356 cites W2118526609 @default.
• W2977882356 cites W2119333469 @default.
• W2977882356 cites W2125066647 @default.
• W2977882356 cites W2137252144 @default.
• W2977882356 cites W2142486812 @default.
• W2977882356 cites W2145083705 @default.
• W2977882356 cites W2146290595 @default.
• W2977882356 cites W2146720183 @default.
• W2977882356 cites W2150856909 @default.
• W2977882356 cites W2152310253 @default.
• W2977882356 cites W2158311939 @default.
• W2977882356 cites W2159474015 @default.
• W2977882356 cites W2159729657 @default.
• W2977882356 cites W2161226469 @default.
• W2977882356 cites W2163924952 @default.
• W2977882356 cites W2165675499 @default.
• W2977882356 cites W2171676623 @default.
• W2977882356 cites W2230276206 @default.
• W2977882356 cites W2252512272 @default.
• W2977882356 cites W2256002492 @default.
• W2977882356 cites W2283933088 @default.
• W2977882356 cites W2303043072 @default.
• W2977882356 cites W2313533128 @default.
• W2977882356 cites W2411785919 @default.
• W2977882356 cites W2429940891 @default.
• W2977882356 cites W2476905916 @default.
• W2977882356 cites W2496524973 @default.
• W2977882356 cites W2561267978 @default.
• W2977882356 cites W2564576803 @default.
• W2977882356 cites W2586766361 @default.
• W2977882356 cites W2612976070 @default.
• W2977882356 cites W2617193254 @default.
• W2977882356 cites W2620606831 @default.
• W2977882356 cites W2709327628 @default.
• W2977882356 cites W2783562720 @default.
• W2977882356 cites W2790016534 @default.
• W2977882356 cites W2803008577 @default.
• W2977882356 cites W2900725275 @default.

• next