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• W2977885645 endingPage "C1204" @default.
• W2977885645 startingPage "C1194" @default.
• W2977885645 abstract "The retinal pigment epithelium (RPE) supports the outer retina through essential roles in the retinoid cycle, nutrient supply, ion exchange, and waste removal. Each day the RPE removes the oldest ~10% of photoreceptor outer segment (OS) disk membranes through phagocytic uptake, which peaks following light onset. Impaired degradation of phagocytosed OS material by the RPE can lead to toxic accumulation of lipids, oxidative tissue damage, inflammation, and cell death. OSs are rich in very long chain fatty acids, which are preferentially catabolized in peroxisomes. Despite the importance of lipid degradation in RPE function, the regulation of peroxisome number and activity relative to diurnal OS ingestion is relatively unexplored. Using immunohistochemistry, immunoblot analysis, and catalase activity assays, we investigated peroxisome abundance and activity at 6 AM, 7 AM (light onset), 8 AM, and 3 PM, in wild-type (WT) mice and mice lacking microtubule-associated protein 1 light chain 3B ( Lc3b), which have impaired phagosome degradation. We found that catalase activity, but not the amount of catalase protein, is 50% higher in the morning compared with 3 PM, in RPE of WT, but not Lc3b −/− , mice. Surprisingly, we found that peroxisome abundance was stable during the day in RPE of WT mice; however, numbers were elevated overall in Lc3b −/− mice, implicating LC3B in autophagic organelle turnover in RPE. Our data suggest that RPE peroxisome function is regulated in coordination with phagocytosis, possibly through direct enzyme regulation, and may serve to prepare RPE peroxisomes for daily surges in ingested lipid-rich OS." @default.
• W2977885645 created "2019-10-10" @default.
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• W2977885645 date "2019-12-01" @default.
• W2977885645 modified "2023-10-18" @default.
• W2977885645 title "Peroxisome turnover and diurnal modulation of antioxidant activity in retinal pigment epithelia utilizes microtubule-associated protein 1 light chain 3B (LC3B)" @default.
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• W2977885645 doi "https://doi.org/10.1152/ajpcell.00185.2019" @default.
• W2977885645 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6962520" @default.
• W2977885645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31577510" @default.
• W2977885645 hasPublicationYear "2019" @default.
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