Matches in SemOpenAlex for { <https://semopenalex.org/work/W2977891789> ?p ?o ?g. }
- W2977891789 endingPage "1636" @default.
- W2977891789 startingPage "1628" @default.
- W2977891789 abstract "Cigarette smoke extract (CSE) contains many toxicants and may derange the physiological processes, such as cholesterol metabolism. We examined the impact of CSE on transcriptional regulation mediated peroxisome proliferator-activated receptors (PPARs) and its interaction with cofactors to elucidate differences in the molecular mechanism between CSE and other agonists of PPARs. We constructed several mutant PPARs (mPPARs) with amino acid substitution in the ligand-binding domain, which according to the molecular modeling, may affect the binding of agonists. In transient expression assays, each wild-type peroxisome proliferator-activated receptor (PPAR) mediated transcription stimulated by CSE was faintly yet significantly elevated compared to the control. The CSE-induced transcriptional activation was abolished in the H323A, H323Y, S342A, and H449A mPPARγs, although the activation elevated by pioglitazone was reserved. In the mPPARγ with Y473A and mPPARβ/δs with H286Y and Y436A, the pioglitazone-induced or L165041-activated transcriptional elevations were decreased and were lower than that of CSE-induced stimulation. These results suggested that CSE activated both mutant PPARs to be selectively different from those ligands. Mammalian two-hybrid assay illustrated that CSE could mildly recruit SRC1 or GRIP1 to the wild-type PPARγ. Representative ingredients, such as acrolein and crotonaldehyde present in CSE, could stimulate PPAR isoforms even at the toxicological concentrations and might possibly contribute to stimulatory effects. CSE mildly regulates the cholesterol metabolism-related genes, such as low density lipoprotein (LDL) receptor and Liver X receptor (LXR)β. In conclusion, these CSE effects the nuclear hormone receptors and their cofactors thereby disturbing metabolic phenomena. Therefore, CSE might be involved in cholesterol metabolism." @default.
- W2977891789 created "2019-10-10" @default.
- W2977891789 creator A5000827667 @default.
- W2977891789 creator A5012388881 @default.
- W2977891789 creator A5035337239 @default.
- W2977891789 creator A5039919959 @default.
- W2977891789 creator A5045879272 @default.
- W2977891789 creator A5047200294 @default.
- W2977891789 creator A5053204186 @default.
- W2977891789 creator A5063319129 @default.
- W2977891789 creator A5076363373 @default.
- W2977891789 creator A5085246285 @default.
- W2977891789 creator A5090573809 @default.
- W2977891789 date "2019-10-01" @default.
- W2977891789 modified "2023-09-27" @default.
- W2977891789 title "Cigarette Smoke Extract Modulates Functions of Peroxisome Proliferator-Activated Receptors" @default.
- W2977891789 cites W1989601885 @default.
- W2977891789 cites W1994649839 @default.
- W2977891789 cites W2003509687 @default.
- W2977891789 cites W2014180706 @default.
- W2977891789 cites W2034071735 @default.
- W2977891789 cites W2040757906 @default.
- W2977891789 cites W2047866054 @default.
- W2977891789 cites W2051609458 @default.
- W2977891789 cites W2078911852 @default.
- W2977891789 cites W2081846665 @default.
- W2977891789 cites W2082460839 @default.
- W2977891789 cites W2107459349 @default.
- W2977891789 cites W2113456624 @default.
- W2977891789 cites W2117533325 @default.
- W2977891789 cites W2123559176 @default.
- W2977891789 cites W2129697991 @default.
- W2977891789 cites W2132388439 @default.
- W2977891789 cites W2138331081 @default.
- W2977891789 cites W2141642527 @default.
- W2977891789 cites W2144962038 @default.
- W2977891789 cites W2145270527 @default.
- W2977891789 cites W2154108206 @default.
- W2977891789 cites W2239563340 @default.
- W2977891789 cites W2261329950 @default.
- W2977891789 cites W2312874913 @default.
- W2977891789 cites W2385633365 @default.
- W2977891789 cites W2407820986 @default.
- W2977891789 cites W2408962679 @default.
- W2977891789 cites W2419296355 @default.
- W2977891789 cites W2746379258 @default.
- W2977891789 cites W2789488401 @default.
- W2977891789 cites W2789493171 @default.
- W2977891789 cites W63594597 @default.
- W2977891789 cites W88344415 @default.
- W2977891789 cites W1882789395 @default.
- W2977891789 cites W2023125021 @default.
- W2977891789 doi "https://doi.org/10.1248/bpb.b18-00991" @default.
- W2977891789 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31582651" @default.
- W2977891789 hasPublicationYear "2019" @default.
- W2977891789 type Work @default.
- W2977891789 sameAs 2977891789 @default.
- W2977891789 citedByCount "0" @default.
- W2977891789 crossrefType "journal-article" @default.
- W2977891789 hasAuthorship W2977891789A5000827667 @default.
- W2977891789 hasAuthorship W2977891789A5012388881 @default.
- W2977891789 hasAuthorship W2977891789A5035337239 @default.
- W2977891789 hasAuthorship W2977891789A5039919959 @default.
- W2977891789 hasAuthorship W2977891789A5045879272 @default.
- W2977891789 hasAuthorship W2977891789A5047200294 @default.
- W2977891789 hasAuthorship W2977891789A5053204186 @default.
- W2977891789 hasAuthorship W2977891789A5063319129 @default.
- W2977891789 hasAuthorship W2977891789A5076363373 @default.
- W2977891789 hasAuthorship W2977891789A5085246285 @default.
- W2977891789 hasAuthorship W2977891789A5090573809 @default.
- W2977891789 hasBestOaLocation W29778917891 @default.
- W2977891789 hasConcept C104317684 @default.
- W2977891789 hasConcept C126322002 @default.
- W2977891789 hasConcept C127078168 @default.
- W2977891789 hasConcept C134018914 @default.
- W2977891789 hasConcept C170493617 @default.
- W2977891789 hasConcept C185592680 @default.
- W2977891789 hasConcept C187345961 @default.
- W2977891789 hasConcept C188413054 @default.
- W2977891789 hasConcept C195598365 @default.
- W2977891789 hasConcept C2777180221 @default.
- W2977891789 hasConcept C2778384471 @default.
- W2977891789 hasConcept C55493867 @default.
- W2977891789 hasConcept C555293320 @default.
- W2977891789 hasConcept C63932345 @default.
- W2977891789 hasConcept C71924100 @default.
- W2977891789 hasConcept C86339819 @default.
- W2977891789 hasConcept C86803240 @default.
- W2977891789 hasConceptScore W2977891789C104317684 @default.
- W2977891789 hasConceptScore W2977891789C126322002 @default.
- W2977891789 hasConceptScore W2977891789C127078168 @default.
- W2977891789 hasConceptScore W2977891789C134018914 @default.
- W2977891789 hasConceptScore W2977891789C170493617 @default.
- W2977891789 hasConceptScore W2977891789C185592680 @default.
- W2977891789 hasConceptScore W2977891789C187345961 @default.
- W2977891789 hasConceptScore W2977891789C188413054 @default.
- W2977891789 hasConceptScore W2977891789C195598365 @default.
- W2977891789 hasConceptScore W2977891789C2777180221 @default.