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- W2977903412 endingPage "120433" @default.
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- W2977903412 abstract "Metal-organic frameworks (MOFs) have been gaining extensive popularity in the field of sample pretreatment due to their designable structures and easy-modifiable surfaces. Herein, zirconium-based MOFs UiO-66-NH2 is first coated with graphene oxide (GO) layer to enhance the stability, and then grafted with hydrophilic polyethylene glycol (PEG) via amidation reaction to give a product of hydrophilic MOF-based nanomaterial [email protected]@PEG. The obtained nanoparticles exhibit typical hydrophilic interaction liquid chromatography (HILIC) behavior towards glycoproteins, making itself powerful adsorbent for the extraction of glycoproteins from complex matrices. Under pH 9.0, an adsorption capacity of 139.6 mg g−1 for glycoprotein immunoglobulin G (IgG) is achieved, and the retained IgG could be recovered by 0.5% (m/v) ammonium hydroxide (NH4OH) as stripping reagent with a recovery rate of 82.6%. Moreover, the [email protected]@PEG shows excellent reusability in IgG isolation. The practicability of [email protected]@PEG is verified by the selective isolation of IgG from human serum, demonstrating the purity of isolated IgG with SDS-PAGE assay." @default.
- W2977903412 created "2019-10-10" @default.
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- W2977903412 date "2020-02-01" @default.
- W2977903412 modified "2023-09-25" @default.
- W2977903412 title "PEGylation of metal-organic framework for selective isolation of glycoprotein immunoglobulin G" @default.
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- W2977903412 doi "https://doi.org/10.1016/j.talanta.2019.120433" @default.
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