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- W2978021212 abstract "Cells in organisms are surrounded by fluid environment, and the fluid shear force produced by this fluid affects the morphology and behaviour of cells. The stimulation of cells in a bioreactor is a key area of research in tissue engineering and regenerative medicine. This paper introduces a fluid shear system for cell culture in vitro. The main component of system is a perfusion chamber, which consists of an inoculation room and a perfusion cap. The inoculation room is used for the common laboratory consumables of microorganism or cell culture, such as petri dish, six-well plate and so on. It can realize to six independent experiments of fluid shear; the new type of perfusion cap was designed to obtain a reasonable cavity by hydrodynamic simulation. The cap was produced by casting technology. It not only has the function of cap with nozzles, but also has the function of sealing. With peristaltic pumps, nozzles, liquid storage bottles and so on, the fluid shearing system which can provide uniform, controllable and up to 60 dyn/cm2 shear stress along the bottom surface is constructed, and is easy to operate and can be personalized." @default.
- W2978021212 created "2019-10-10" @default.
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- W2978021212 date "2019-09-01" @default.
- W2978021212 modified "2023-09-26" @default.
- W2978021212 title "Development of a New Fluid Shearing System for Cell Culture in Vitro" @default.
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- W2978021212 doi "https://doi.org/10.1088/1757-899x/626/1/012013" @default.
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