Matches in SemOpenAlex for { <https://semopenalex.org/work/W2978092416> ?p ?o ?g. }
- W2978092416 abstract "Abstract The DNA double helix is unwound by the Cdc45/Mcm2-7/GINS (CMG) complex at the eukaryotic replication fork. While isolated CMG unwinds duplex DNA very slowly, its fork unwinding rate is stimulated by an order of magnitude by single-stranded DNA binding protein, RPA. However, the molecular mechanism by which RPA enhances CMG helicase activity remained elusive. Here, we demonstrate that engagement of CMG with parental double-stranded DNA (dsDNA) at the replication fork impairs its helicase activity, explaining the slow DNA unwinding by isolated CMG. Using single-molecule and ensemble biochemistry, we show that binding of RPA to the excluded DNA strand prevents duplex engagement by the helicase and speeds up CMG-mediated DNA unwinding. When stalled due to dsDNA interaction, DNA rezipping-induced helicase backtracking re-establishes productive helicase-fork engagement underscoring the significance of plasticity in helicase action. Together, our results elucidate the dynamics of CMG at the replication fork and reveal how other replisome components can mediate proper DNA engagement by the replicative helicase to achieve efficient fork progression." @default.
- W2978092416 created "2019-10-10" @default.
- W2978092416 creator A5020034146 @default.
- W2978092416 creator A5029279606 @default.
- W2978092416 creator A5044275356 @default.
- W2978092416 creator A5050936358 @default.
- W2978092416 creator A5081789765 @default.
- W2978092416 date "2019-10-07" @default.
- W2978092416 modified "2023-09-26" @default.
- W2978092416 title "Mechanism of RPA-Facilitated Processive DNA Unwinding by the Eukaryotic CMG Helicase" @default.
- W2978092416 cites W1508702670 @default.
- W2978092416 cites W1985491895 @default.
- W2978092416 cites W1986663423 @default.
- W2978092416 cites W1986968571 @default.
- W2978092416 cites W1987086578 @default.
- W2978092416 cites W1999925341 @default.
- W2978092416 cites W2007214324 @default.
- W2978092416 cites W2008424177 @default.
- W2978092416 cites W2008486131 @default.
- W2978092416 cites W2008628928 @default.
- W2978092416 cites W2020328641 @default.
- W2978092416 cites W2038946742 @default.
- W2978092416 cites W2041008570 @default.
- W2978092416 cites W2044440192 @default.
- W2978092416 cites W2047998400 @default.
- W2978092416 cites W2063402277 @default.
- W2978092416 cites W2071149713 @default.
- W2978092416 cites W2075601360 @default.
- W2978092416 cites W2085416172 @default.
- W2978092416 cites W2090845358 @default.
- W2978092416 cites W2094381752 @default.
- W2978092416 cites W2096597528 @default.
- W2978092416 cites W2099487576 @default.
- W2978092416 cites W2105413529 @default.
- W2978092416 cites W2105656306 @default.
- W2978092416 cites W2106741497 @default.
- W2978092416 cites W2109470020 @default.
- W2978092416 cites W2121296152 @default.
- W2978092416 cites W2122867782 @default.
- W2978092416 cites W2124221060 @default.
- W2978092416 cites W2126918645 @default.
- W2978092416 cites W2138154707 @default.
- W2978092416 cites W2144130189 @default.
- W2978092416 cites W2158074805 @default.
- W2978092416 cites W2161289356 @default.
- W2978092416 cites W2279843283 @default.
- W2978092416 cites W2316204282 @default.
- W2978092416 cites W2559896842 @default.
- W2978092416 cites W2562393208 @default.
- W2978092416 cites W2578571972 @default.
- W2978092416 cites W2600668326 @default.
- W2978092416 cites W2736809432 @default.
- W2978092416 cites W2752723776 @default.
- W2978092416 cites W2754218973 @default.
- W2978092416 cites W2757357164 @default.
- W2978092416 cites W2766127952 @default.
- W2978092416 cites W2771250096 @default.
- W2978092416 cites W2787975097 @default.
- W2978092416 cites W2791015842 @default.
- W2978092416 cites W2885236691 @default.
- W2978092416 cites W2891374857 @default.
- W2978092416 cites W2912332641 @default.
- W2978092416 cites W2916972625 @default.
- W2978092416 cites W2922261746 @default.
- W2978092416 cites W2946514347 @default.
- W2978092416 cites W2964264171 @default.
- W2978092416 cites W2971471877 @default.
- W2978092416 doi "https://doi.org/10.1101/796003" @default.
- W2978092416 hasPublicationYear "2019" @default.
- W2978092416 type Work @default.
- W2978092416 sameAs 2978092416 @default.
- W2978092416 citedByCount "0" @default.
- W2978092416 crossrefType "posted-content" @default.
- W2978092416 hasAuthorship W2978092416A5020034146 @default.
- W2978092416 hasAuthorship W2978092416A5029279606 @default.
- W2978092416 hasAuthorship W2978092416A5044275356 @default.
- W2978092416 hasAuthorship W2978092416A5050936358 @default.
- W2978092416 hasAuthorship W2978092416A5081789765 @default.
- W2978092416 hasBestOaLocation W29780924161 @default.
- W2978092416 hasConcept C104317684 @default.
- W2978092416 hasConcept C107827726 @default.
- W2978092416 hasConcept C113271649 @default.
- W2978092416 hasConcept C116107829 @default.
- W2978092416 hasConcept C12554922 @default.
- W2978092416 hasConcept C149187182 @default.
- W2978092416 hasConcept C153911025 @default.
- W2978092416 hasConcept C156719811 @default.
- W2978092416 hasConcept C161223559 @default.
- W2978092416 hasConcept C175248279 @default.
- W2978092416 hasConcept C194830204 @default.
- W2978092416 hasConcept C54355233 @default.
- W2978092416 hasConcept C552990157 @default.
- W2978092416 hasConcept C58167604 @default.
- W2978092416 hasConcept C66072924 @default.
- W2978092416 hasConcept C67705224 @default.
- W2978092416 hasConcept C68592252 @default.
- W2978092416 hasConcept C73573662 @default.
- W2978092416 hasConcept C86339819 @default.
- W2978092416 hasConcept C86803240 @default.
- W2978092416 hasConcept C94966510 @default.