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- W2978314354 abstract "As to long term intracellular parasitism,the genome reduction of microsporidia was significant.We surveyed the genome data of Nosema bombycis to identify whether there is any coding sequence acting as the promoter sequence in such significantly reduced genome.We found that there are typical characteristics of the eukaryotic promoter in the N-terminal coding sequence of cell division cycle protein(CDCP) which plays an important role in regulation of DNA transcription.We designed specific primers to amplify this gene's N-terminal 590 bp which contained the promoter motif sequence(C promoter sequence).Subsequently,we cloned it into the pUG35 vector of which the promoter(MET25-P)was replaced with the termination sequence CYC1-T to construct the vector ΔpUG35-CYC1-T-C-yEGFP-CYC1-T that had the 590 bp promoter sequence to activate expression of its downstream green fluorescent protein(GFP).We transformed this vector into the Saccharomyces cerevisiae CEN.PK2 strain by electroporation,and obtained the positive clone.After screening and induction culture,fluorescence microscopic observation showed signals of GFP expression,verifying that the N-terminal coding sequence of cell division cycle protein of N.bombycis has promoter activity.This phenomenon indicates that some coding sequences could act as regulatory elements in the reduced genome of N.bombycis.Our work also provides a simple method to test the promoter activity of microsporidia." @default.
- W2978314354 created "2019-10-10" @default.
- W2978314354 creator A5046927622 @default.
- W2978314354 date "2011-01-01" @default.
- W2978314354 modified "2023-09-23" @default.
- W2978314354 title "The N-terminal Coding Sequence of Cell Division Cycle Protein of Nosema bombycis has Promoter Activity in Saccharomyces cerevisiae" @default.
- W2978314354 hasPublicationYear "2011" @default.
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