Matches in SemOpenAlex for { <https://semopenalex.org/work/W2978362904> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2978362904 abstract "Abstract The N-terminal regions of histone proteins (tails) are dynamic elements that protrude from the nucleosome and are involved in many aspects of chromatin organization. Their epigenetic role is well-established, and post-translational modifications (PTMs) present on these regions contribute to transcriptional regulation. While hydrogen/deuterium exchange mass spectrometry (HX-MS) is well-suited for the analysis of dynamic structures, it has seldom been employed to analyze histones due to the poor N-terminal coverage obtained using pepsin. Here, we test the applicability of a dual protease type XIII/pepsin digestion column to this class of proteins. We optimize online digestion conditions using the H4 monomer, and extend the method to the analysis of histones in monomeric states and nucleosome core particles (NCPs). We show that the dual protease column generates many short and overlapping N-terminal peptides. We evaluate our method by performing hydrogen exchange experiments of NCPs for different time points and present full coverage of the tails at excellent resolution. We further employ electron transfer dissociation (ETD) and showcase an unprecedented degree of overlap across multiple peptides that is several fold higher than previously reported methods. The method we report here may be readily applied to the HX-MS investigation of histone dynamics and to the footprints of histone binding proteins on nucleosomes." @default.
- W2978362904 created "2019-10-10" @default.
- W2978362904 creator A5006801972 @default.
- W2978362904 creator A5010743762 @default.
- W2978362904 creator A5036255260 @default.
- W2978362904 creator A5048651737 @default.
- W2978362904 creator A5056665352 @default.
- W2978362904 creator A5071189138 @default.
- W2978362904 date "2019-10-01" @default.
- W2978362904 modified "2023-09-27" @default.
- W2978362904 title "“Dual protease type XIII/pepsin digestion offers superior resolution and overlap for the analysis of histone tails by HX-MS”" @default.
- W2978362904 cites W1975203212 @default.
- W2978362904 cites W2002698073 @default.
- W2978362904 cites W2010048885 @default.
- W2978362904 cites W2010579992 @default.
- W2978362904 cites W2041336616 @default.
- W2978362904 cites W2067899389 @default.
- W2978362904 cites W2084334588 @default.
- W2978362904 cites W2084370050 @default.
- W2978362904 cites W2088793589 @default.
- W2978362904 cites W2119979674 @default.
- W2978362904 cites W2130479394 @default.
- W2978362904 cites W2131741443 @default.
- W2978362904 cites W2135351891 @default.
- W2978362904 cites W2136518039 @default.
- W2978362904 cites W2144792857 @default.
- W2978362904 cites W2314833185 @default.
- W2978362904 cites W2557776755 @default.
- W2978362904 cites W2606309635 @default.
- W2978362904 cites W2894739207 @default.
- W2978362904 cites W2898900539 @default.
- W2978362904 doi "https://doi.org/10.1101/788463" @default.
- W2978362904 hasPublicationYear "2019" @default.
- W2978362904 type Work @default.
- W2978362904 sameAs 2978362904 @default.
- W2978362904 citedByCount "0" @default.
- W2978362904 crossrefType "posted-content" @default.
- W2978362904 hasAuthorship W2978362904A5006801972 @default.
- W2978362904 hasAuthorship W2978362904A5010743762 @default.
- W2978362904 hasAuthorship W2978362904A5036255260 @default.
- W2978362904 hasAuthorship W2978362904A5048651737 @default.
- W2978362904 hasAuthorship W2978362904A5056665352 @default.
- W2978362904 hasAuthorship W2978362904A5071189138 @default.
- W2978362904 hasBestOaLocation W29783629041 @default.
- W2978362904 hasConcept C12554922 @default.
- W2978362904 hasConcept C162356407 @default.
- W2978362904 hasConcept C166940927 @default.
- W2978362904 hasConcept C167569220 @default.
- W2978362904 hasConcept C178790620 @default.
- W2978362904 hasConcept C185592680 @default.
- W2978362904 hasConcept C2777109674 @default.
- W2978362904 hasConcept C31827203 @default.
- W2978362904 hasConcept C43617362 @default.
- W2978362904 hasConcept C521977710 @default.
- W2978362904 hasConcept C552990157 @default.
- W2978362904 hasConcept C55493867 @default.
- W2978362904 hasConcept C64927066 @default.
- W2978362904 hasConcept C70721500 @default.
- W2978362904 hasConcept C83640560 @default.
- W2978362904 hasConcept C84772758 @default.
- W2978362904 hasConcept C86803240 @default.
- W2978362904 hasConceptScore W2978362904C12554922 @default.
- W2978362904 hasConceptScore W2978362904C162356407 @default.
- W2978362904 hasConceptScore W2978362904C166940927 @default.
- W2978362904 hasConceptScore W2978362904C167569220 @default.
- W2978362904 hasConceptScore W2978362904C178790620 @default.
- W2978362904 hasConceptScore W2978362904C185592680 @default.
- W2978362904 hasConceptScore W2978362904C2777109674 @default.
- W2978362904 hasConceptScore W2978362904C31827203 @default.
- W2978362904 hasConceptScore W2978362904C43617362 @default.
- W2978362904 hasConceptScore W2978362904C521977710 @default.
- W2978362904 hasConceptScore W2978362904C552990157 @default.
- W2978362904 hasConceptScore W2978362904C55493867 @default.
- W2978362904 hasConceptScore W2978362904C64927066 @default.
- W2978362904 hasConceptScore W2978362904C70721500 @default.
- W2978362904 hasConceptScore W2978362904C83640560 @default.
- W2978362904 hasConceptScore W2978362904C84772758 @default.
- W2978362904 hasConceptScore W2978362904C86803240 @default.
- W2978362904 hasLocation W29783629041 @default.
- W2978362904 hasOpenAccess W2978362904 @default.
- W2978362904 hasPrimaryLocation W29783629041 @default.
- W2978362904 hasRelatedWork W2059028670 @default.
- W2978362904 hasRelatedWork W2062514148 @default.
- W2978362904 hasRelatedWork W2099114692 @default.
- W2978362904 hasRelatedWork W2121105652 @default.
- W2978362904 hasRelatedWork W2344991865 @default.
- W2978362904 hasRelatedWork W2415288070 @default.
- W2978362904 hasRelatedWork W2434482954 @default.
- W2978362904 hasRelatedWork W2570932838 @default.
- W2978362904 hasRelatedWork W2911205078 @default.
- W2978362904 hasRelatedWork W4296162192 @default.
- W2978362904 isParatext "false" @default.
- W2978362904 isRetracted "false" @default.
- W2978362904 magId "2978362904" @default.
- W2978362904 workType "article" @default.