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- W2978401042 abstract "Objective To study how to design T7-promotor-Oligo-DNAs and compose short-hairpin RNA then evaluate the effect of RNA interference that used T7-ShRNAs transfer SKOV3 cells and correlative study with sensitivity of chemotherapy(CBP).Methods Designed the oligo-DNA template which contains T7-promotor according to C-erbB2 exons,designed the hairpin sequence.Then used it to evaluate the inhibitory effection of shRNA,after we transfect it into SK-OV3 cells.Used immuno-histo-chemistry、semi-quantitation PCR to evaluate the the inhibitory effection,then Apply CCK-8 to count the cell growth dynamic so that observe the inhibitory effection and sensitivity of chemotherapy(CBP).Results RNAi result by RT-PCR、immuno-histo-chemistry cells print and CCK-8:inhibiting rate shRNAb shRNAa(P0.01),it's no effect in shRNAc、no-transfect group and irrespective-RNA group;after 24 h shRNAa's inhibiting was resumed,it was get right at 72 h,that shRNAb's inhibiting effection is remarkable,especially in 48 h;even at 72 h,its inhibitory effection was retained,when RNAi associated with CBP the inhibitory effection was most remarkable.Conclusion shRNAb's inhibiting is successfully,and it is possible to use siRNA to effectly interfere the expression of C-erbB2 in tumor cells so that settle the base that RNAi can made gene dysfunction,and ameliorate the sensitivity of chemotherapy(CBP)." @default.
- W2978401042 created "2019-10-10" @default.
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- W2978401042 date "2008-01-01" @default.
- W2978401042 modified "2023-09-24" @default.
- W2978401042 title "Correlative study to specific silencing (RNAi) by T7-shRNAs and sensitivity of chemotherapy in ovarian cancer" @default.
- W2978401042 hasPublicationYear "2008" @default.
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