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- W2978441641 abstract "Relaxases are essential proteins for plasmid conjugation. They process the DNA to be transferred by means of a covalent intermediate. Thus, the characterization of these covalent complexes is essential to understand the biological role of this reaction and to improve it for biotechnological applications. In this article, we describe the use of the polyacrylamide electrophoresis techniques for the identification of relaxase-DNA covalent complexes, being SDS-PAGE a simple and reliable method for the detection of protein-DNA covalent adducts. Relaxases also perform a strand transfer reaction to recircularize the DNA and finish the DNA transfer process in the recipient cell. Urea-PAGE allows us the analysis of oligonucleotides generated by the strand transfer reaction. These methods could also be used for the analysis of other HUH endonucleases." @default.
- W2978441641 created "2019-10-10" @default.
- W2978441641 creator A5054646768 @default.
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- W2978441641 date "2019-10-05" @default.
- W2978441641 modified "2023-10-17" @default.
- W2978441641 title "Identification of Relaxase-DNA Covalent Complexes and DNA Strand Transfer Reaction Products by Polyacrylamide Gel Electrophoresis" @default.
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- W2978441641 doi "https://doi.org/10.1007/978-1-4939-9877-7_11" @default.
- W2978441641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31584161" @default.
- W2978441641 hasPublicationYear "2019" @default.
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