Matches in SemOpenAlex for { <https://semopenalex.org/work/W2978589610> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W2978589610 endingPage "1765" @default.
- W2978589610 startingPage "1762" @default.
- W2978589610 abstract "Objective To address the effects of osteopontin (OPN) on hypercalciuria contributing to the kidney stone formation.Methods The normal rat kidney epithelial cell line NRK cells were divided into three groups,which were cultured in the medium containing calcium of 0.1,0.3 and 0.6 g/L,respectively.The cell proliferation,the content of OPN in the cell culture supernatants and the level of OPN gene expression at 3,6 and 24 h were examined.The lentivirus vector targeting OPN was constructed.The NRK cells were infected with the lentivirus and then cultured in the medium containing calcium of 0.6 g/L for 24 h.Eventually,The inhibitory effects of silencing OPN gene on the adhesion of calcium oxalate crystals to NRK cells were investigated.Results High level calcium could significantly suppress the proliferation of NRK cells and stimulate secretion of OPN as compared with the blank control group (P ＜ 0.05).The expression of OPN mRNA and protein was also significantly increased (P ＜ 0.05).Calcium concentrations of the lysate of infected NRK cells in the three groups after calcium chloride stimulation were (19.25 ±3.71),(27.22 ±3.82) and (31.91 ± 1.15) mg/L,respectively.The silencing of OPN gene expression could significantly inhibit the abilities of attachment of calcium oxalate crystals to NRK cells as compared with negative lentivirus vector and blank control group,respectively (P ＜ 0.05).Conclusion High level calcium could induce the adhesion of calcium oxalate crystals to NRK cells probably mediated by OPN.Key words: Hypercalciuria ; Osteopontin; Renal epithelial cell" @default.
- W2978589610 created "2019-10-10" @default.
- W2978589610 creator A5031900566 @default.
- W2978589610 creator A5043029022 @default.
- W2978589610 creator A5045215814 @default.
- W2978589610 creator A5052762267 @default.
- W2978589610 creator A5053930709 @default.
- W2978589610 creator A5058207955 @default.
- W2978589610 creator A5059272754 @default.
- W2978589610 creator A5080921329 @default.
- W2978589610 date "2014-08-08" @default.
- W2978589610 modified "2023-09-23" @default.
- W2978589610 title "Effects of osteopontin on hypercalciuria-induced attachment of calcium oxalate crystals to rat renal epithelial cells" @default.
- W2978589610 doi "https://doi.org/10.3760/cma.j.issn.1001-9030.2014.08.052" @default.
- W2978589610 hasPublicationYear "2014" @default.
- W2978589610 type Work @default.
- W2978589610 sameAs 2978589610 @default.
- W2978589610 citedByCount "0" @default.
- W2978589610 crossrefType "journal-article" @default.
- W2978589610 hasAuthorship W2978589610A5031900566 @default.
- W2978589610 hasAuthorship W2978589610A5043029022 @default.
- W2978589610 hasAuthorship W2978589610A5045215814 @default.
- W2978589610 hasAuthorship W2978589610A5052762267 @default.
- W2978589610 hasAuthorship W2978589610A5053930709 @default.
- W2978589610 hasAuthorship W2978589610A5058207955 @default.
- W2978589610 hasAuthorship W2978589610A5059272754 @default.
- W2978589610 hasAuthorship W2978589610A5080921329 @default.
- W2978589610 hasConcept C126322002 @default.
- W2978589610 hasConcept C134018914 @default.
- W2978589610 hasConcept C153911025 @default.
- W2978589610 hasConcept C178790620 @default.
- W2978589610 hasConcept C185592680 @default.
- W2978589610 hasConcept C2776179656 @default.
- W2978589610 hasConcept C2776351790 @default.
- W2978589610 hasConcept C2777724520 @default.
- W2978589610 hasConcept C2780091579 @default.
- W2978589610 hasConcept C2780804394 @default.
- W2978589610 hasConcept C519063684 @default.
- W2978589610 hasConcept C54355233 @default.
- W2978589610 hasConcept C71924100 @default.
- W2978589610 hasConcept C81885089 @default.
- W2978589610 hasConcept C86803240 @default.
- W2978589610 hasConceptScore W2978589610C126322002 @default.
- W2978589610 hasConceptScore W2978589610C134018914 @default.
- W2978589610 hasConceptScore W2978589610C153911025 @default.
- W2978589610 hasConceptScore W2978589610C178790620 @default.
- W2978589610 hasConceptScore W2978589610C185592680 @default.
- W2978589610 hasConceptScore W2978589610C2776179656 @default.
- W2978589610 hasConceptScore W2978589610C2776351790 @default.
- W2978589610 hasConceptScore W2978589610C2777724520 @default.
- W2978589610 hasConceptScore W2978589610C2780091579 @default.
- W2978589610 hasConceptScore W2978589610C2780804394 @default.
- W2978589610 hasConceptScore W2978589610C519063684 @default.
- W2978589610 hasConceptScore W2978589610C54355233 @default.
- W2978589610 hasConceptScore W2978589610C71924100 @default.
- W2978589610 hasConceptScore W2978589610C81885089 @default.
- W2978589610 hasConceptScore W2978589610C86803240 @default.
- W2978589610 hasIssue "08" @default.
- W2978589610 hasLocation W29785896101 @default.
- W2978589610 hasOpenAccess W2978589610 @default.
- W2978589610 hasPrimaryLocation W29785896101 @default.
- W2978589610 hasRelatedWork W2069963870 @default.
- W2978589610 hasRelatedWork W2282835293 @default.
- W2978589610 hasRelatedWork W2325037965 @default.
- W2978589610 hasRelatedWork W2350546520 @default.
- W2978589610 hasRelatedWork W2354089251 @default.
- W2978589610 hasRelatedWork W2356572126 @default.
- W2978589610 hasRelatedWork W2357828280 @default.
- W2978589610 hasRelatedWork W2360716368 @default.
- W2978589610 hasRelatedWork W2363335410 @default.
- W2978589610 hasRelatedWork W2367037922 @default.
- W2978589610 hasRelatedWork W2388918605 @default.
- W2978589610 hasRelatedWork W2389803343 @default.
- W2978589610 hasRelatedWork W2391595819 @default.
- W2978589610 hasRelatedWork W2392703735 @default.
- W2978589610 hasRelatedWork W3030516773 @default.
- W2978589610 hasRelatedWork W3030958036 @default.
- W2978589610 hasRelatedWork W3031718537 @default.
- W2978589610 hasRelatedWork W3094724802 @default.
- W2978589610 hasRelatedWork W3166650878 @default.
- W2978589610 hasRelatedWork W2983152747 @default.
- W2978589610 hasVolume "31" @default.
- W2978589610 isParatext "false" @default.
- W2978589610 isRetracted "false" @default.
- W2978589610 magId "2978589610" @default.
- W2978589610 workType "article" @default.