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- W2978602510 abstract "Objective To prepare rabbit antibody against mouse AD-004 by AD-004 expressed in the prokaryotic expression system and to identify its distribution in the testis and adrenal. Methods The full-length cDNA of mouse AD-004 was cloned into PET28 plasmid, and the protein was induced in E. coli BL21 bacteria by adding IPTG and then purified by Ni2+ -NTA column. The purified protein was used as an immunogene to prepare polyclonal" @default.
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- W2978602510 date "2006-08-25" @default.
- W2978602510 modified "2023-09-25" @default.
- W2978602510 title "Protein expression and preparation of polyclonal antibody of AD-004 and study on its expression in the adrenal and testis" @default.
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