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- W2978604833 abstract "Objective To discuss the possibilities of the intracellular delivery of CTL epitope by cell penetrating peptide HIV Tat 49-57 and of the ensuing MHC class Ⅰ pathway presentation. Methods A nonamer (P1) peptide (MART 1 27-35 ) of CTL epitote and an 18 mer peptide (P2) containing HIV Tat 49-57 and MART 1 27-35 were synthesized with aid of polypeptide solid phase synthesis technique. The intracellular transport capabilities of these peptides were tested with indirect immunofluorescence assay and confocal microscopy. In addition, the nonamer and 18 mer peptide were used to stimulate PBMC of the healthy HLA A2.1+ blood donors. Then, the activity of MART 1 27-35 specific CTL in PBMC of those donors was measured by using a standard 4 h 51 Gr release assay. Results The 18mer peptide was more effective than the nonamer in cellular uptake and efficiently crossed the plasma membranes of the live cells in a time and concentration-dependent fashion. Furthermore, the specific CTL activity induced by the 18mer was much stronger than that induced by the nonamer. Conclusion The results show that HIV Tat 49-57 can deliver the exogenous antigenic peptide into the cytoplasm of live cells and probably trigger the MHC class Ⅰ processing pathway. Thus, the described procedure should be applied to the design of peptide vaccine in order to induce CTL immunity against tumor and intracellular infection." @default.
- W2978604833 created "2019-10-10" @default.
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- W2978604833 date "2002-01-01" @default.
- W2978604833 modified "2023-09-25" @default.
- W2978604833 title "Immunologic effect of a new peptide vaccine containing CTL epitope transferred by cell-penetrating peptide HIV-Tat_(49-57)" @default.
- W2978604833 hasPublicationYear "2002" @default.
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