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• W2978621880 abstract "Abstract Buruli ulcer is an emerging-necrotizing skin infection, responsible for permanent deformity if untreated, caused by the pathogen Mycobacterium ulcerans ( M. ulcerans ). Despite this debilitating condition, no specific disease-modifying therapeutics or vaccination is available. Therefore, we aimed to design an effective multi-epitope vaccine against M. ulcerans through an integrated vaccinomics approach. Briefly, the highest antigenic PE-PGRS protein was selected from which the promiscuous T- and B-cell epitopes were predicted. After rigorous assessment, 15 promising CTL, HTL and LBL epitopes were selected. The identified T-cell epitopes showed marked interactions towards the HLA binding alleles and provided 99.8% world population coverage. Consequently, a vaccine chimera was designed by connecting these epitopes with suitable linkers and adjuvant (LprG). The vaccine construct was antigenic and immunogenic as well as non-allergenic; hence, subjected to homology modelling. The molecular docking and dynamic simulation revealed strong and stable binding affinity between the vaccine and TLR2 receptor. The binding energy (ΔG) and dissociation constant (K d ) were −15.3 kcal/mol and 5.9×10 −12 M, respectively. Further, disulfide engineering was applied to improve vaccine’ stability and higher expression in Escherichia coli K12 system was ensured by codon optimization and cloning in silico . The computer-simulated immune responses were characterized by higher levels of IgM and IgG antibodies, helper T-cells with increased IFN-γ production, and macrophage activity crucial for immunity against M. ulcerans . Therefore, our data suggest that, if the designed vaccine is validated experimentally, it will prevent Buruli ulcer by generating robust immune response against M. ulcerans ." @default.
• W2978621880 created "2019-10-10" @default.
• W2978621880 creator A5001810848 @default.
• W2978621880 creator A5016039756 @default.
• W2978621880 creator A5026524483 @default.
• W2978621880 creator A5045198947 @default.
• W2978621880 date "2019-10-07" @default.
• W2978621880 modified "2023-09-28" @default.
• W2978621880 title "Structural Basis and Designing of Peptide Vaccine using PE-PGRS Family Protein of Mycobacterium ulcerans – An Integrated Vaccinomics Approach" @default.
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