FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2978689014> ?p ?o ?g. }

• W2978689014 endingPage "59" @default.
• W2978689014 startingPage "51" @default.
• W2978689014 abstract "Abstract Background LpxB is an enzyme involved in the biosynthesis pathway of lipid A, a component of LPS. Objectives To evaluate the lpxB gene in Acinetobacter baumannii as a potential therapeutic target and to propose antisense agents such as peptide nucleic acids (PNAs) as a tool to combat bacterial infection, either alone or in combination with known antimicrobial therapies. Methods RNA-seq analysis of the A. baumannii ATCC 17978 strain in a murine pneumonia model was performed to study the in vivo expression of lpxB. Protein expression was studied in the presence or absence of anti-lpxB (KFF)3K-PNA (pPNA). Time–kill curve analyses and protection assays of infected A549 cells were performed. The chequerboard technique was used to test for synergy between pPNA and colistin. A Galleria mellonella infection model was used to test the in vivo efficacy of pPNA. Results The lpxB gene was overexpressed during pneumonia. Treatment with a specific pPNA inhibited LpxB expression in vitro, decreased survival of the ATCC 17978 strain and increased the survival rate of infected A549 cells. Synergy was observed between pPNA and colistin in colistin-susceptible strains. In vivo assays confirmed that a combination treatment of anti-lpxB pPNA and colistin was more effective than colistin in monotherapy. Conclusions The lpxB gene is essential for A. baumannii survival. Anti-lpxB pPNA inhibits LpxB expression, causing bacterial death. This pPNA showed synergy with colistin and increased the survival rate in G. mellonella. The data suggest that antisense pPNA molecules blocking the lpxB gene could be used as antibacterial agents." @default.
• W2978689014 created "2019-10-10" @default.
• W2978689014 creator A5024666798 @default.
• W2978689014 creator A5028518636 @default.
• W2978689014 creator A5044232361 @default.
• W2978689014 creator A5044676398 @default.
• W2978689014 creator A5050160466 @default.
• W2978689014 creator A5082548129 @default.
• W2978689014 creator A5084803025 @default.
• W2978689014 date "2019-10-05" @default.
• W2978689014 modified "2023-09-26" @default.
• W2978689014 title "Antisense inhibition of lpxB gene expression in Acinetobacter baumannii by peptide–PNA conjugates and synergy with colistin" @default.
• W2978689014 cites W1165233182 @default.
• W2978689014 cites W1514259902 @default.
• W2978689014 cites W1532766628 @default.
• W2978689014 cites W1553897125 @default.
• W2978689014 cites W1563786107 @default.
• W2978689014 cites W1935760988 @default.
• W2978689014 cites W1967346383 @default.
• W2978689014 cites W1975313581 @default.
• W2978689014 cites W1982581071 @default.
• W2978689014 cites W1992995635 @default.
• W2978689014 cites W1997684437 @default.
• W2978689014 cites W1999033641 @default.
• W2978689014 cites W2006167464 @default.
• W2978689014 cites W2006881751 @default.
• W2978689014 cites W2008060328 @default.
• W2978689014 cites W2016562927 @default.
• W2978689014 cites W2019818589 @default.
• W2978689014 cites W2034248364 @default.
• W2978689014 cites W2046245796 @default.
• W2978689014 cites W2054499506 @default.
• W2978689014 cites W2061015821 @default.
• W2978689014 cites W2066959898 @default.
• W2978689014 cites W2071159951 @default.
• W2978689014 cites W2079062605 @default.
• W2978689014 cites W2089249139 @default.
• W2978689014 cites W2095615218 @default.
• W2978689014 cites W2104713605 @default.
• W2978689014 cites W2105838577 @default.
• W2978689014 cites W2115879450 @default.
• W2978689014 cites W2120593627 @default.
• W2978689014 cites W2121148907 @default.
• W2978689014 cites W2126504345 @default.
• W2978689014 cites W2138675768 @default.
• W2978689014 cites W2140348754 @default.
• W2978689014 cites W2142343447 @default.
• W2978689014 cites W2147706925 @default.
• W2978689014 cites W2159435953 @default.
• W2978689014 cites W2163623511 @default.
• W2978689014 cites W2166723759 @default.
• W2978689014 cites W2291393495 @default.
• W2978689014 cites W2569210251 @default.
• W2978689014 cites W2594873912 @default.
• W2978689014 cites W2773471937 @default.
• W2978689014 cites W2783017631 @default.
• W2978689014 cites W2785360699 @default.
• W2978689014 cites W2798072540 @default.
• W2978689014 cites W2909495700 @default.
• W2978689014 cites W2909653722 @default.
• W2978689014 cites W2948382142 @default.
• W2978689014 cites W2968479826 @default.
• W2978689014 cites W4234317413 @default.
• W2978689014 cites W4255507877 @default.
• W2978689014 cites W4293241248 @default.
• W2978689014 doi "https://doi.org/10.1093/jac/dkz409" @default.
• W2978689014 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31586411" @default.
• W2978689014 hasPublicationYear "2019" @default.
• W2978689014 type Work @default.
• W2978689014 sameAs 2978689014 @default.
• W2978689014 citedByCount "16" @default.
• W2978689014 countsByYear W29786890142020 @default.
• W2978689014 countsByYear W29786890142021 @default.
• W2978689014 countsByYear W29786890142023 @default.
• W2978689014 crossrefType "journal-article" @default.
• W2978689014 hasAuthorship W2978689014A5024666798 @default.
• W2978689014 hasAuthorship W2978689014A5028518636 @default.
• W2978689014 hasAuthorship W2978689014A5044232361 @default.
• W2978689014 hasAuthorship W2978689014A5044676398 @default.
• W2978689014 hasAuthorship W2978689014A5050160466 @default.
• W2978689014 hasAuthorship W2978689014A5082548129 @default.
• W2978689014 hasAuthorship W2978689014A5084803025 @default.
• W2978689014 hasConcept C2776315533 @default.
• W2978689014 hasConcept C2777637488 @default.
• W2978689014 hasConcept C2779259085 @default.
• W2978689014 hasConcept C501593827 @default.
• W2978689014 hasConcept C523546767 @default.
• W2978689014 hasConcept C54355233 @default.
• W2978689014 hasConcept C86803240 @default.
• W2978689014 hasConcept C89423630 @default.
• W2978689014 hasConceptScore W2978689014C2776315533 @default.
• W2978689014 hasConceptScore W2978689014C2777637488 @default.
• W2978689014 hasConceptScore W2978689014C2779259085 @default.
• W2978689014 hasConceptScore W2978689014C501593827 @default.
• W2978689014 hasConceptScore W2978689014C523546767 @default.
• W2978689014 hasConceptScore W2978689014C54355233 @default.
• W2978689014 hasConceptScore W2978689014C86803240 @default.
• W2978689014 hasConceptScore W2978689014C89423630 @default.

• next