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- W2978805870 abstract "Vibrio fortis is a pathogenic bacteria of sea urchin Strongylocentrotus intermedius.To establish PCR detection process of V.fortis,three pairs of primer VF-6,VF-7 and VF-8 were designed according to the specified region of gyrB genes of V.fortis.20 strains of bacteria were included in PCR amplification as control.The results showed that the amplified fragments of PCR applied with the three pairs of primer were all agreed with the expected sizes,and no PCR product was found from the other reference strains.Different dilutions of bacterial suspension were used as DNA templates in PCR amplification,the results showed that the three pairs of primer was found to detect the minimum bacterial concentration of 4.1×104,4.1×103,4.1×102 cfu/mL respectively.Meanwhile,different dilutions of DNA were performed in PCR amplification,the results showed that the minimum detectable concentration of bacteria DNA was 1.4,0.14,0.014 pg/μL respectively.These experiments showed that all the three pairs of primers demonstrated excellent specificity to V.fortis.However,the sensitivity of the PCR amplifications was different among the three primers,PCR procedure applied with primer VF-8 showing the best.V.fortis in coelomic fluid of sea urchin and the environmental samples were detected by PCR detection method with VF-8.The results of healthy sea urchin,breeding seawater and feeding kelp were negative,while the sample of seawater from natural sea area had a certain amount of V.fortis." @default.
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- W2978805870 date "2012-01-01" @default.
- W2978805870 modified "2023-09-27" @default.
- W2978805870 title "PCR detection of pathogen Vibrio fortis in sea urchin Strongylocentrotu intermedius" @default.
- W2978805870 hasPublicationYear "2012" @default.
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