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- W2979029764 abstract "Purpose: Crohn's disease (CD) is a chronic inflammatory disorder of the gastrointestinal tract. The T lymphocytes of CD patients are associated with increased Th1/Th17 cytokines. The immune system down-regulates unwanted inflammatory responses through specialized subpopulations of T lymphocytes, namely T-regulatory lymphocytes (Treg; CD127-, CD25+). The Treg lymphocytes can be subdivided into resting Treg, activated Treg and activated conventional T lymphocytes. Only resting and activated Treg (rTreg and aTreg) lymphocytes have suppressive activity. Objective: To conduct a cross-sectional evaluation of children with CD to 1) assess the frequency of rTreg and aTreg lymphocytes, and 2) identify CCR7 (C-C chemokine receptor type 7) expressing, CD45RA+ effector memory (EMRA) T lymphocytes as candidate disease initiating T lymphocytes. Methods: Eleven children with CD were enrolled in the study. There were eight males and three females. The mean age was 15 years and 7 months. Ten normal controls are derived from normal pediatric bone marrow transplant donors. Blood samples were collected during clinic visit and/or hospitalization. Their disease activities were divided into mild (n=7), or moderate to severe (n=4) based on the PCDAI scores. Treg lymphocytes were immunophenotypically assessed using the Miyara classification by 6-color immunofluorescence on a CANTO FACS machine. A sequential gating strategy was used. Results are presented as a percentage of total Treg lymphocytes. The identification of candidate disease initiating T lymphocytes included identifying EMRA T lymphocytes since the disease initiating T lymphocytes are hypothesized to be present in the memory T lymphocyte subpopulations. Results: rTreg and aTreg lymphocytes of the CD patients with mild disease were similar to controls (frequency of 25.8 and 2.1 to those of normal individuals of 16.9 and 1.5). However, children with moderate to severe CD showed lower frequency of rTreg (57% of normal) and similar aTreg (112% of normal) lymphocytes. EMRA T lymphocytes (CD45RA+, CD62L-) were increased (264% of normal) in CD patients (frequency of 14.3 when compared to 3.4 of normal individuals). The increased EMRA was much greater (911% of normal) in CD patients with moderate to severe disease activity (frequency of 31.0 as compared to 3.4 of normal individuals). Conclusion: Our findings demonstrated that children with moderate to severe CD have decreased rTreg lymphocytes. Our data also showed highly increased EMRA (candidate disease initiating T lymphocytes) in children with Crohn's disease. Our findings of Treg dysfunction in moderate to severe CD may provide an alternative treatment approach of pediatric CD (i.e., in vivo boosting of Treg cell number/function)." @default.
- W2979029764 created "2019-10-10" @default.
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- W2979029764 date "2013-10-01" @default.
- W2979029764 modified "2023-09-26" @default.
- W2979029764 title "Suppressed Resting Regulatory T Lymphocytes in Children with Moderate to Severe Crohnʼs Disease: Presidential Poster" @default.
- W2979029764 doi "https://doi.org/10.14309/00000434-201310001-02058" @default.
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