FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2979180825> ?p ?o ?g. }

• W2979180825 endingPage "5538" @default.
• W2979180825 startingPage "5525" @default.
• W2979180825 abstract "Bioengineered skeletal muscle tissues benefit from dynamic culture environments which facilitate the appropriate provision of nutrients and removal of cellular waste products. Biologically compatible perfusion systems hold the potential to enhance the physiological biomimicry of in vitro tissues via dynamic culture, in addition to providing technological advances in analytical testing and live cellular imaging for analysis of cellular development. To meet such diverse requirements, perfusion systems require the capacity and adaptability to incorporate multiple cell laden constructs of both monolayer and bioengineered tissues. This work reports perfusion systems produced using additive manufacturing technology for the in situ phenotypic development of myogenic precursor cells in monolayer and bioengineered tissue. Biocompatibility of systems 3D printed using stereolithography (SL), laser sintering (LS), and PolyJet outlined preferential morphological development within both SL and LS devices. When exposed to intermittent perfusion in the monolayer, delayed yet physiologically representative cellular proliferation, MyoD and myogenin transcription of C2C12 cells was evident. Long-term (8 days) intermittent perfusion of monolayer cultures outlined viable morphological and genetic in situ differentiation for the live cellular imaging of myogenic development. Continuous perfusion cultures (13 days) of bioengineered skeletal muscle tissues outlined in situ myogenic differentiation, forming mature multinucleated myotubes. Here, reductions in IL-1β and TNF-α inflammatory cytokines, myostatin, and MuRF-1 atrophic mRNA expression were observed. Comparable myosin heavy chain (MyHC) isoform transcription profiles were evident between conditions; however, total mRNA expression was reduced in perfusion conditions. Decreased transcription of MuRF1 and subsequent reduced ubiquitination of the MyHC protein allude to a decreased requirement for transcription of MyHC isoform transcripts. Together, these data appear to indicate that 3D printed perfusion systems elicit enhanced stability of the culture environment, resulting in a reduced basal requirement for MyHC gene expression within bioengineered skeletal muscle tissue." @default.
• W2979180825 created "2019-10-10" @default.
• W2979180825 creator A5008705797 @default.
• W2979180825 creator A5019636931 @default.
• W2979180825 creator A5035437164 @default.
• W2979180825 creator A5036491164 @default.
• W2979180825 creator A5044353895 @default.
• W2979180825 creator A5076487472 @default.
• W2979180825 creator A5084138706 @default.
• W2979180825 creator A5086096850 @default.
• W2979180825 date "2019-10-03" @default.
• W2979180825 modified "2023-10-17" @default.
• W2979180825 title "Differentiation of Bioengineered Skeletal Muscle within a 3D Printed Perfusion Bioreactor Reduces Atrophic and Inflammatory Gene Expression" @default.
• W2979180825 cites W1532909280 @default.
• W2979180825 cites W1891826212 @default.
• W2979180825 cites W1969626676 @default.
• W2979180825 cites W1971726857 @default.
• W2979180825 cites W1973963945 @default.
• W2979180825 cites W1976896311 @default.
• W2979180825 cites W1985015512 @default.
• W2979180825 cites W1985145039 @default.
• W2979180825 cites W1985534077 @default.
• W2979180825 cites W1993266458 @default.
• W2979180825 cites W1995746991 @default.
• W2979180825 cites W1997524040 @default.
• W2979180825 cites W2003670269 @default.
• W2979180825 cites W2004441496 @default.
• W2979180825 cites W2005840935 @default.
• W2979180825 cites W2005997252 @default.
• W2979180825 cites W2017472601 @default.
• W2979180825 cites W2026192447 @default.
• W2979180825 cites W2032618829 @default.
• W2979180825 cites W2033797333 @default.
• W2979180825 cites W2033924360 @default.
• W2979180825 cites W2035555824 @default.
• W2979180825 cites W2038933145 @default.
• W2979180825 cites W2042136385 @default.
• W2979180825 cites W2048338339 @default.
• W2979180825 cites W2052651806 @default.
• W2979180825 cites W2059725783 @default.
• W2979180825 cites W2060028261 @default.
• W2979180825 cites W2061929833 @default.
• W2979180825 cites W2077717135 @default.
• W2979180825 cites W2078088548 @default.
• W2979180825 cites W2078718449 @default.
• W2979180825 cites W2078863581 @default.
• W2979180825 cites W2086514035 @default.
• W2979180825 cites W2088183084 @default.
• W2979180825 cites W2095126612 @default.
• W2979180825 cites W2100688137 @default.
• W2979180825 cites W2103037575 @default.
• W2979180825 cites W2106883683 @default.
• W2979180825 cites W2107277218 @default.
• W2979180825 cites W2108930270 @default.
• W2979180825 cites W2111199691 @default.
• W2979180825 cites W2120629822 @default.
• W2979180825 cites W2125169325 @default.
• W2979180825 cites W2127912123 @default.
• W2979180825 cites W2133675750 @default.
• W2979180825 cites W2137035181 @default.
• W2979180825 cites W2137812881 @default.
• W2979180825 cites W2139623037 @default.
• W2979180825 cites W2144203293 @default.
• W2979180825 cites W2150387617 @default.
• W2979180825 cites W2152118403 @default.
• W2979180825 cites W2153217835 @default.
• W2979180825 cites W2155581711 @default.
• W2979180825 cites W2158095398 @default.
• W2979180825 cites W2160534216 @default.
• W2979180825 cites W2166044024 @default.
• W2979180825 cites W2167033587 @default.
• W2979180825 cites W2167231187 @default.
• W2979180825 cites W2167279371 @default.
• W2979180825 cites W2168607364 @default.
• W2979180825 cites W2171103133 @default.
• W2979180825 cites W2171726169 @default.
• W2979180825 cites W2181293815 @default.
• W2979180825 cites W2193696010 @default.
• W2979180825 cites W2225233485 @default.
• W2979180825 cites W2314139499 @default.
• W2979180825 cites W2362504809 @default.
• W2979180825 cites W2475325728 @default.
• W2979180825 cites W2496296245 @default.
• W2979180825 cites W2553880037 @default.
• W2979180825 cites W2571908772 @default.
• W2979180825 cites W2585876527 @default.
• W2979180825 cites W2594447998 @default.
• W2979180825 cites W2607417071 @default.
• W2979180825 cites W2621842261 @default.
• W2979180825 cites W2739646612 @default.
• W2979180825 cites W2739789506 @default.
• W2979180825 cites W2752973615 @default.
• W2979180825 cites W2753231520 @default.
• W2979180825 cites W2761967374 @default.
• W2979180825 cites W2781902139 @default.
• W2979180825 cites W2808656548 @default.
• W2979180825 cites W2911311020 @default.
• W2979180825 cites W4230884859 @default.

• next