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- W2979290223 abstract "Purpose The immobilized glutamate decarboxylase(IGAD)was prepared for produing γ-aminobutyric acid(GABA).Methods Glutamate decarboxylase(GAD)was embedded-immobilized with sodium alginate and gelatin as the blending supports.Factors which affected the activity of IGAD were studied and optimum conditions were investigated.Afterwards,IGAD was applied for GABA transformation.Results 65.3% enzyme activity of IGAD could be reserved compared with unimmobilized one when the concentration of sodium alginate,gelatin,CaCl2,glutaraldehyde and solidifying time were 2.5%,1.0%,3.0%,0.3% and 4 h respectively.Afterwards,the immobilized enzyme was filled in packed bed reactor for producing GABA continuously.After 60 h reacting,the reserved enzyme activity was kept as high as 76.9% of the initial,and the transform rate of glutamate reached to 98.7%.Conclusion This IGAD can be applied for GABA biotransformation continuously." @default.
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- W2979290223 date "2007-01-01" @default.
- W2979290223 modified "2023-09-26" @default.
- W2979290223 title "Research on immobilized glutamate decarboxylase for γ-aminobutyric acid transformation" @default.
- W2979290223 hasPublicationYear "2007" @default.
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