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- W2979316963 abstract "Abstract The extreme potency of the plant toxin, ricin, is due to its enzymatic subunit, RTA, which inactivates mammalian ribosomes with near perfect efficiency. Here we characterized, at the functional and structural levels, seven alpaca single-domain antibodies (V H Hs) previously reported to recognize epitopes in proximity to RTA’s active site. Three of the V H Hs, V2A11, V8E6 and V2G10, were potent inhibitors of RTA in vitro and protected Vero cells from ricin when expressed as intracellular antibodies (“intrabodies”). Crystal structure analysis revealed that the complementarity-determining region 3 (CDR3) elements of V2A11 and V8E6 penetrate RTA’s active site and interact with key catalytic residues. V2G10, in contrast, sits atop the enzymatic pocket and occludes substrate accessibility. The other four V H Hs also penetrated/occluded RTA’s active site, but lacked sufficient binding affinities to outcompete RTA-ribosome interactions. Intracellular delivery of high-affinity, single-domain antibodies may offer a new avenue in the development of countermeasures against ricin toxin." @default.
- W2979316963 created "2019-10-18" @default.
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- W2979316963 date "2019-10-15" @default.
- W2979316963 modified "2023-10-18" @default.
- W2979316963 title "Intracellular Neutralization of Ricin Toxin by Single Domain Antibodies Targeting the Active Site Pocket" @default.
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- W2979316963 doi "https://doi.org/10.1101/805754" @default.
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