FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2979359232> ?p ?o ?g. }

• W2979359232 endingPage "5722" @default.
• W2979359232 startingPage "5722" @default.
• W2979359232 abstract "Abstract Multipotent mesenchymal stromal cells (MSCs) have immunomodulatory properties and have been successfully used for treatment of autoimmune diseases and acute or chronic graft-versus-host disease. Therapy with MSCs is not always effective. It has been shown that MSCs immunomodulatory properties can be improved by means of various agents, such as IFN-g, TNF-a, IL-17. After 4 hours of IFN-g exposure the expression level of immunomodulatory genes increased - IDO1 300, CSF1 - 7, and IL6 - 2.4 times. MSCs typically express low levels of MHC class I, and no MHC class II or co-stimulatory molecules (e.g., B7-1, B7-2, or CD40), making them partially immunoprivileged. However, treatment with IFN-g leads to increased expression of HLA-DR antigens on MSCs. After injection to the patient the characteristics of MSCs differ from those which have been studied in culture due to their interactions with other cells in the bloodstream and tissues. In this study the model of MSCs and MSCs treated with IFN-g (IFN-g-MSC) interactions with allogeneic lymphocytes in vitro was developed. The aim of the study was to identify the changes in MSCs and IFN-g-MSCs characteristics after co-cultivation with lymphocytes in vitro in dynamics. Materials and methods MSCs were isolated from 13 bone marrow (BM) samples used for allogeneic hematopoietic cells transplantation and cultured by a standard method in aMEM with 10% fetal bovine serum (FBS). MSCs on 2-3-d passages were seeded 105 cells per flask with 25 cm2 bottom area and a day later 500 units/mL of IFN-g were added for 4 hours to half of the cultures. Then the media was changed on RPMI-1640 with 10% FBS. Some cultures were seeded with 106 allogeneic lymphocytes, to half of these cultures 5 mg/ml phytohemagglutinin (PHA) was added for lymphocytes activation. All flasks were cultured up to 4 days at 37°C and 5% CO2. After 1, 2, 3 and 4 days lymphocytes were washed from MSCs. MSCs were removed from the flasks with trypsin and the number of viable cells was determined by dye exclusion method (trypan blue). For each of the MSCs cultures the mean fluorescent signal intensity level (MFI) of HLA-DR was determined by direct immunofluorescent staining with anti-HLA-DR APC (BD Pharmingen) antibodies and measured on flow cytometer BD FACS Canto II (BD Biosciences, USA). Data are presented as mean ± standard error. Statistical analysis was performed using Student's t-test (considered reliable p <0.05). Results The number of cells in cultures of MSCs and IFN-g-MSCs did not differ significantly during the 4 days of observations. The presence of non-activated lymphocytes had no effect on the parameters of growth and viability of MSCs. Co-cultivation of activated lymphocytes with MSCs resulted in a reduction of viable MSCs, to 51.6 ± 5.5% compared with IFN-g-MSCs (68,1 ± 6,2%, p = 0.02). The viability of MSCs cultures without lymphocytes was 74.5% ± 4.8 on day 4 from the beginning of the experiment. HLA-DR expression level gradually increased in IFN-g-MSCs (see table). Co-culturing MSCs with lymphocytes also leads to a gradual increase in HLA-DR expression on MSCs apparently due to IFN-g, produced by lymphocytes. However, in IFN-g-MSCs co-cultured with lymphocytes HLA-DR MFI was significantly lower (p = 0.05) than without lymphocytes. HLA-DR MFI increased about 10 times in MSCs co-cultured with activated lymphocytes regardless of their IFN-g pretreatment The data obtained indicate that IFN-g-MSCs did not change in their growth characteristics and had increased immunomodulating properties. IFN-g-MSCs have a greater resistance to activated lymphocytes, which makes them more effective for cell therapy. The results suggest that injected to patient MSCs could have increased level of HLA-DR expression regardless of their initial treatment with IFN-g. The increase of HLA-DR expression on MSCs co-cultured with lymphocytes indicates a possibility of loss of immune privilege by these cells when injected to patient. Disclosures No relevant conflicts of interest to declare." @default.
• W2979359232 created "2019-10-18" @default.
• W2979359232 creator A5008276842 @default.
• W2979359232 creator A5014421357 @default.
• W2979359232 creator A5016258184 @default.
• W2979359232 creator A5019917735 @default.
• W2979359232 creator A5023711660 @default.
• W2979359232 creator A5030043766 @default.
• W2979359232 creator A5032496170 @default.
• W2979359232 creator A5055461919 @default.
• W2979359232 creator A5089672253 @default.
• W2979359232 date "2016-12-02" @default.
• W2979359232 modified "2023-09-26" @default.
• W2979359232 title "Immune Privileged Features of Multipotent Mesenchymal Stromal Cells Are Lost after Co-Cultivation with Allogeneic Lymphocytes in Vitro" @default.
• W2979359232 doi "https://doi.org/10.1182/blood.v128.22.5722.5722" @default.
• W2979359232 hasPublicationYear "2016" @default.
• W2979359232 type Work @default.
• W2979359232 sameAs 2979359232 @default.
• W2979359232 citedByCount "0" @default.
• W2979359232 crossrefType "journal-article" @default.
• W2979359232 hasAuthorship W2979359232A5008276842 @default.
• W2979359232 hasAuthorship W2979359232A5014421357 @default.
• W2979359232 hasAuthorship W2979359232A5016258184 @default.
• W2979359232 hasAuthorship W2979359232A5019917735 @default.
• W2979359232 hasAuthorship W2979359232A5023711660 @default.
• W2979359232 hasAuthorship W2979359232A5030043766 @default.
• W2979359232 hasAuthorship W2979359232A5032496170 @default.
• W2979359232 hasAuthorship W2979359232A5055461919 @default.
• W2979359232 hasAuthorship W2979359232A5089672253 @default.
• W2979359232 hasConcept C109159458 @default.
• W2979359232 hasConcept C126322002 @default.
• W2979359232 hasConcept C154317977 @default.
• W2979359232 hasConcept C198826908 @default.
• W2979359232 hasConcept C202751555 @default.
• W2979359232 hasConcept C203014093 @default.
• W2979359232 hasConcept C2780007613 @default.
• W2979359232 hasConcept C28328180 @default.
• W2979359232 hasConcept C2911091166 @default.
• W2979359232 hasConcept C39347974 @default.
• W2979359232 hasConcept C502942594 @default.
• W2979359232 hasConcept C55493867 @default.
• W2979359232 hasConcept C71924100 @default.
• W2979359232 hasConcept C86803240 @default.
• W2979359232 hasConcept C8891405 @default.
• W2979359232 hasConcept C95444343 @default.
• W2979359232 hasConceptScore W2979359232C109159458 @default.
• W2979359232 hasConceptScore W2979359232C126322002 @default.
• W2979359232 hasConceptScore W2979359232C154317977 @default.
• W2979359232 hasConceptScore W2979359232C198826908 @default.
• W2979359232 hasConceptScore W2979359232C202751555 @default.
• W2979359232 hasConceptScore W2979359232C203014093 @default.
• W2979359232 hasConceptScore W2979359232C2780007613 @default.
• W2979359232 hasConceptScore W2979359232C28328180 @default.
• W2979359232 hasConceptScore W2979359232C2911091166 @default.
• W2979359232 hasConceptScore W2979359232C39347974 @default.
• W2979359232 hasConceptScore W2979359232C502942594 @default.
• W2979359232 hasConceptScore W2979359232C55493867 @default.
• W2979359232 hasConceptScore W2979359232C71924100 @default.
• W2979359232 hasConceptScore W2979359232C86803240 @default.
• W2979359232 hasConceptScore W2979359232C8891405 @default.
• W2979359232 hasConceptScore W2979359232C95444343 @default.
• W2979359232 hasIssue "22" @default.
• W2979359232 hasLocation W29793592321 @default.
• W2979359232 hasOpenAccess W2979359232 @default.
• W2979359232 hasPrimaryLocation W29793592321 @default.
• W2979359232 hasRelatedWork W1970182741 @default.
• W2979359232 hasRelatedWork W1971444449 @default.
• W2979359232 hasRelatedWork W1975612301 @default.
• W2979359232 hasRelatedWork W1991654720 @default.
• W2979359232 hasRelatedWork W2058607765 @default.
• W2979359232 hasRelatedWork W2168514322 @default.
• W2979359232 hasRelatedWork W2348035871 @default.
• W2979359232 hasRelatedWork W2358518505 @default.
• W2979359232 hasRelatedWork W2794757297 @default.
• W2979359232 hasRelatedWork W4322762817 @default.
• W2979359232 hasVolume "128" @default.
• W2979359232 isParatext "false" @default.
• W2979359232 isRetracted "false" @default.
• W2979359232 magId "2979359232" @default.
• W2979359232 workType "article" @default.