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• W2979394417 abstract "Abstract An investigation of the yeast cell resealing process was performed by studying the absorption of the tetraphenylphosphonium (TPP + ) ion by the yeast Saccharomyces cerevisiae . It was shown that the main barrier for the uptake of such TPP + ions is the cell wall. An increased rate of TPP + absorption after treatment of such cells with a pulsed electric field (PEF) was observed only in intact cells, but not in spheroplasts. The investigation of the uptake of TPP + in PEF treated cells exposed to TPP + for different time intervals also showed the dependence of the absorption rate on the PEF strength. The modelling of the TPP + uptake recovery has also shown that the characteristic decay time of the non-equilibrium (PEF induced) pores was approximately a few tens of seconds and this did not depend on the PEF strength. A further investigation of such cell membrane recovery process using a florescent SYTOX Green nucleic acid stain dye also showed that such membrane resealing takes place over a time that is like that occurring in the cell wall. It was thus concluded that the similar characteristic lifetimes of the non-equilibrium pores in the cell wall and membrane after exposure to PEF indicate a strong coupling between these parts of the cell." @default.
• W2979394417 created "2019-10-18" @default.
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• W2979394417 date "2019-10-14" @default.
• W2979394417 modified "2023-10-14" @default.
• W2979394417 title "The link between yeast cell wall porosity and plasma membrane permeability after PEF treatment" @default.
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• W2979394417 doi "https://doi.org/10.1038/s41598-019-51184-y" @default.
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