Matches in SemOpenAlex for { <https://semopenalex.org/work/W2979566712> ?p ?o ?g. }
- W2979566712 abstract "Polymyxin antibiotics are a last-line treatment for multidrug-resistant Gram-negative bacteria. However, the emergence of colistin resistance, including the spread of mobile mcr genes, necessitates the development of improved diagnostics for the detection of colistin-resistant organisms in hospital settings. The recently developed MALDIxin test enables detection of colistin resistance by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) in less than 15 min but is not optimized for the mass spectrometers commonly found in clinical microbiology laboratories. In this study, we adapted the MALDIxin test for the MALDI Biotyper Sirius MALDI-TOF MS system (Bruker Daltonics). We optimized the sample preparation protocol by using a set of 6 mobile colistin resistance (MCR) protein-expressing Escherichia coli clones and validated the assay with a collection of 40 E. coli clinical isolates, including 19 confirmed MCR protein producers, 12 colistin-resistant isolates that tested negative for commonly encountered mcr genes (i.e., likely chromosomally resistant isolates), and 9 polymyxin-susceptible isolates. We calculated polymyxin resistance ratio (PRR) values from the acquired spectra; PRR values of 0, indicating polymyxin susceptibility, were obtained for all colistin-susceptible E. coli isolates, whereas positive PRR values, indicating resistance to polymyxins, were obtained for all resistant strains, independent of the genetic basis of resistance. Thus, we report a preliminary feasibility study showing that an optimized version of the MALDIxin test adapted for the routine MALDI Biotyper Sirius system provides an unbiased, fast, reliable, cost-effective, and high-throughput way of detecting colistin resistance in clinical E. coli isolates." @default.
- W2979566712 created "2019-10-18" @default.
- W2979566712 creator A5000053096 @default.
- W2979566712 creator A5013294175 @default.
- W2979566712 creator A5015929268 @default.
- W2979566712 creator A5025141902 @default.
- W2979566712 creator A5031492621 @default.
- W2979566712 creator A5035086338 @default.
- W2979566712 creator A5037361850 @default.
- W2979566712 creator A5043370339 @default.
- W2979566712 creator A5048848554 @default.
- W2979566712 creator A5088997870 @default.
- W2979566712 date "2019-12-01" @default.
- W2979566712 modified "2023-10-16" @default.
- W2979566712 title "Detection of Colistin Resistance in Escherichia coli by Use of the MALDI Biotyper Sirius Mass Spectrometry System" @default.
- W2979566712 cites W1606853044 @default.
- W2979566712 cites W1993850849 @default.
- W2979566712 cites W2116245384 @default.
- W2979566712 cites W2117795567 @default.
- W2979566712 cites W2179036997 @default.
- W2979566712 cites W2183577566 @default.
- W2979566712 cites W2583196822 @default.
- W2979566712 cites W2593444269 @default.
- W2979566712 cites W2622029395 @default.
- W2979566712 cites W2801149479 @default.
- W2979566712 cites W2803249533 @default.
- W2979566712 cites W2810427827 @default.
- W2979566712 cites W2888805117 @default.
- W2979566712 cites W2899512978 @default.
- W2979566712 cites W2899549732 @default.
- W2979566712 cites W2905492287 @default.
- W2979566712 cites W2913118041 @default.
- W2979566712 cites W2918097643 @default.
- W2979566712 cites W2944063377 @default.
- W2979566712 doi "https://doi.org/10.1128/jcm.01427-19" @default.
- W2979566712 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/6879293" @default.
- W2979566712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31597744" @default.
- W2979566712 hasPublicationYear "2019" @default.
- W2979566712 type Work @default.
- W2979566712 sameAs 2979566712 @default.
- W2979566712 citedByCount "34" @default.
- W2979566712 countsByYear W29795667122020 @default.
- W2979566712 countsByYear W29795667122021 @default.
- W2979566712 countsByYear W29795667122022 @default.
- W2979566712 countsByYear W29795667122023 @default.
- W2979566712 crossrefType "journal-article" @default.
- W2979566712 hasAuthorship W2979566712A5000053096 @default.
- W2979566712 hasAuthorship W2979566712A5013294175 @default.
- W2979566712 hasAuthorship W2979566712A5015929268 @default.
- W2979566712 hasAuthorship W2979566712A5025141902 @default.
- W2979566712 hasAuthorship W2979566712A5031492621 @default.
- W2979566712 hasAuthorship W2979566712A5035086338 @default.
- W2979566712 hasAuthorship W2979566712A5037361850 @default.
- W2979566712 hasAuthorship W2979566712A5043370339 @default.
- W2979566712 hasAuthorship W2979566712A5048848554 @default.
- W2979566712 hasAuthorship W2979566712A5088997870 @default.
- W2979566712 hasBestOaLocation W29795667121 @default.
- W2979566712 hasConcept C104317684 @default.
- W2979566712 hasConcept C2779259085 @default.
- W2979566712 hasConcept C2780026444 @default.
- W2979566712 hasConcept C2780675486 @default.
- W2979566712 hasConcept C2992965680 @default.
- W2979566712 hasConcept C501593827 @default.
- W2979566712 hasConcept C54355233 @default.
- W2979566712 hasConcept C547475151 @default.
- W2979566712 hasConcept C86803240 @default.
- W2979566712 hasConcept C89423630 @default.
- W2979566712 hasConceptScore W2979566712C104317684 @default.
- W2979566712 hasConceptScore W2979566712C2779259085 @default.
- W2979566712 hasConceptScore W2979566712C2780026444 @default.
- W2979566712 hasConceptScore W2979566712C2780675486 @default.
- W2979566712 hasConceptScore W2979566712C2992965680 @default.
- W2979566712 hasConceptScore W2979566712C501593827 @default.
- W2979566712 hasConceptScore W2979566712C54355233 @default.
- W2979566712 hasConceptScore W2979566712C547475151 @default.
- W2979566712 hasConceptScore W2979566712C86803240 @default.
- W2979566712 hasConceptScore W2979566712C89423630 @default.
- W2979566712 hasFunder F4320307874 @default.
- W2979566712 hasFunder F4320334626 @default.
- W2979566712 hasIssue "12" @default.
- W2979566712 hasLocation W29795667121 @default.
- W2979566712 hasLocation W29795667122 @default.
- W2979566712 hasLocation W29795667123 @default.
- W2979566712 hasLocation W29795667124 @default.
- W2979566712 hasLocation W29795667125 @default.
- W2979566712 hasLocation W29795667126 @default.
- W2979566712 hasOpenAccess W2979566712 @default.
- W2979566712 hasPrimaryLocation W29795667121 @default.
- W2979566712 hasRelatedWork W2015989262 @default.
- W2979566712 hasRelatedWork W2038305694 @default.
- W2979566712 hasRelatedWork W2069674352 @default.
- W2979566712 hasRelatedWork W2793858051 @default.
- W2979566712 hasRelatedWork W2796671428 @default.
- W2979566712 hasRelatedWork W2909385855 @default.
- W2979566712 hasRelatedWork W2916950232 @default.
- W2979566712 hasRelatedWork W2965981848 @default.
- W2979566712 hasRelatedWork W3199491928 @default.
- W2979566712 hasRelatedWork W3201223818 @default.
- W2979566712 hasVolume "57" @default.
- W2979566712 isParatext "false" @default.