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• W2979739780 abstract "Critical biomarkers of disease are increasingly being detected by point-of-care assays. Chemiluminescence (CL) and electrochemiluminescence (ECL) are often used in such assays due to their convenience and that they do not require light sources or other components that could complicate or add cost to the system. Reports of these assays often include readers built on a cellphone platform or constructed from low-cost components. However, the impact the optical design has on the limit of detection (LOD) in these systems remains unexamined. Here, we report a theoretical rubric to evaluate different optical designs in terms of maximizing the use of photons emitted from a CL or ECL assay to improve the LOD. We demonstrate that the majority of cellphone designs reported in the literature are not optimized, in part due to misunderstandings of the optical tradeoffs in collection systems, and in part due to limitations imposed on the designs arising from the use of a mobile phone with a very small lens aperture. Based on the theoretical rubric, we design a new portable reader built using off-the-shelf condenser optics, and demonstrate a nearly 10× performance enhancement compared to prior reports on an ECL assays running on a portable chip." @default.
• W2979739780 created "2019-10-18" @default.
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• W2979739780 date "2019-10-23" @default.
• W2979739780 modified "2023-10-14" @default.
• W2979739780 title "Improving the limit of detection in portable luminescent assay readers through smart optical design" @default.
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• W2979739780 doi "https://doi.org/10.1002/jbio.201900241" @default.
• W2979739780 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31602762" @default.
• W2979739780 hasPublicationYear "2019" @default.
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