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- W2979852335 abstract "The 9-aminoacridine (9-AA) damaged lambda gt11DNA was added to Lambda packaging extracts and then the repacked Lambda phage were cultured in E. coli Y1090 on a selective plate pre-supplemented with X-Gal and IPTG. The positive cloned DNA molecule was determined with DNA sequencer. Results: 9-AA had an obvious effect on the survival rate of the Lambda phage and induce DNA mutation in a dose-effect manner. The 9-AA induced not only frameshift mutations, but also base substitutions. The frameshift mutation mainly occurred in the repeats of As, Cs, Gs. and Ts, and the frequency elevated linearly with the increasing amount of As, Cs, Gs, and Ts in the repeat. The 9-AA-induced base substitutions were mainly base transversion, and the base transition was only in Gs repeat." @default.
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- W2979852335 date "2001-01-01" @default.
- W2979852335 modified "2023-09-27" @default.
- W2979852335 title "Molecular mechanism of LacZ gene mutagenesis induced by 9-aminoacridine" @default.
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