FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2979958958> ?p ?o ?g. }

• W2979958958 abstract "Abstract Due to the overlapping emission spectra of fluorophores, fluorescence microscopy images often have bleed-through problems, leading to a false positive detection. This problem is almost unavoidable when the samples are labeled with three or more fluorophores, and the situation is complicated even further when imaged under a multiphoton microscope. Several methods have been developed and commonly used by biologists for fluorescence microscopy spectral unmixing, such as linear unmixing, non-negative matrix factorization, deconvolution, and principal component analysis. However, they either require pre-knowledge of emission spectra or restrict the number of fluorophores to be the same as detection channels, which highly limits the real-world applications of those spectral unmixing methods. In this paper, we developed a robust and flexible spectral unmixing method: Learning Unsupervised Means of Spectra (LUMoS), which uses an unsupervised machine learning clustering method to learn individual fluorophores’ spectral signatures from mixed images, and blindly separate channels without restrictions on the number of fluorophores that can be imaged. This method highly expands the hardware capability of two-photon microscopy to simultaneously image more fluorophores than is possible with instrumentation alone. Experimental and simulated results demonstrated the robustness of LUMoS in multi-channel separations of two-photon microscopy images. We also extended the application of this method to background/autofluorescence removal and colocalization analysis. Lastly, we integrated this tool into ImageJ to offer an easy to use spectral unmixing tool for fluorescence imaging. LUMoS allows us to gain a higher spectral resolution and obtain a cleaner image without the need to upgrade the imaging hardware capabilities." @default.
• W2979958958 created "2019-10-18" @default.
• W2979958958 creator A5001650041 @default.
• W2979958958 creator A5030324050 @default.
• W2979958958 creator A5056929800 @default.
• W2979958958 creator A5078953147 @default.
• W2979958958 date "2019-10-09" @default.
• W2979958958 modified "2023-09-27" @default.
• W2979958958 title "Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning" @default.
• W2979958958 cites W1486131515 @default.
• W2979958958 cites W1487409748 @default.
• W2979958958 cites W1783389077 @default.
• W2979958958 cites W180242331 @default.
• W2979958958 cites W1828367075 @default.
• W2979958958 cites W1902027874 @default.
• W2979958958 cites W1960394152 @default.
• W2979958958 cites W1964570608 @default.
• W2979958958 cites W1970550565 @default.
• W2979958958 cites W1974733207 @default.
• W2979958958 cites W1979198244 @default.
• W2979958958 cites W1984098851 @default.
• W2979958958 cites W198705282 @default.
• W2979958958 cites W1988606601 @default.
• W2979958958 cites W1991219220 @default.
• W2979958958 cites W1999502333 @default.
• W2979958958 cites W2015886513 @default.
• W2979958958 cites W2023226382 @default.
• W2979958958 cites W2041060053 @default.
• W2979958958 cites W2043079694 @default.
• W2979958958 cites W2050027510 @default.
• W2979958958 cites W2058187222 @default.
• W2979958958 cites W2061464631 @default.
• W2979958958 cites W2063623305 @default.
• W2979958958 cites W2065045053 @default.
• W2979958958 cites W2079293985 @default.
• W2979958958 cites W2088617449 @default.
• W2979958958 cites W2095846115 @default.
• W2979958958 cites W2112946281 @default.
• W2979958958 cites W2113504999 @default.
• W2979958958 cites W2118068450 @default.
• W2979958958 cites W2122269389 @default.
• W2979958958 cites W2124680996 @default.
• W2979958958 cites W2126374246 @default.
• W2979958958 cites W2141203194 @default.
• W2979958958 cites W2141397097 @default.
• W2979958958 cites W2143094150 @default.
• W2979958958 cites W2146658490 @default.
• W2979958958 cites W2150530391 @default.
• W2979958958 cites W2150737519 @default.
• W2979958958 cites W2160258952 @default.
• W2979958958 cites W2161932153 @default.
• W2979958958 cites W2162328319 @default.
• W2979958958 cites W2165135104 @default.
• W2979958958 cites W2167279371 @default.
• W2979958958 cites W2169145403 @default.
• W2979958958 cites W2184638288 @default.
• W2979958958 cites W2198668039 @default.
• W2979958958 cites W2228502273 @default.
• W2979958958 cites W2268116636 @default.
• W2979958958 cites W2286236434 @default.
• W2979958958 cites W2296544499 @default.
• W2979958958 cites W2340249672 @default.
• W2979958958 cites W2394629425 @default.
• W2979958958 cites W2465777910 @default.
• W2979958958 cites W2474156463 @default.
• W2979958958 cites W2520303620 @default.
• W2979958958 cites W2580035316 @default.
• W2979958958 cites W2594777858 @default.
• W2979958958 cites W2735968258 @default.
• W2979958958 cites W2740320131 @default.
• W2979958958 cites W2792897399 @default.
• W2979958958 cites W2799548397 @default.
• W2979958958 cites W3099772852 @default.
• W2979958958 cites W4211231550 @default.
• W2979958958 cites W4231254085 @default.
• W2979958958 cites W4233760599 @default.
• W2979958958 doi "https://doi.org/10.1101/797993" @default.
• W2979958958 hasPublicationYear "2019" @default.
• W2979958958 type Work @default.
• W2979958958 sameAs 2979958958 @default.
• W2979958958 citedByCount "0" @default.
• W2979958958 crossrefType "posted-content" @default.
• W2979958958 hasAuthorship W2979958958A5001650041 @default.
• W2979958958 hasAuthorship W2979958958A5030324050 @default.
• W2979958958 hasAuthorship W2979958958A5056929800 @default.
• W2979958958 hasAuthorship W2979958958A5078953147 @default.
• W2979958958 hasBestOaLocation W29799589581 @default.
• W2979958958 hasConcept C120665830 @default.
• W2979958958 hasConcept C121332964 @default.
• W2979958958 hasConcept C147080431 @default.
• W2979958958 hasConcept C153180895 @default.
• W2979958958 hasConcept C154945302 @default.
• W2979958958 hasConcept C159078339 @default.
• W2979958958 hasConcept C169274487 @default.
• W2979958958 hasConcept C174576160 @default.
• W2979958958 hasConcept C176641082 @default.
• W2979958958 hasConcept C179687394 @default.
• W2979958958 hasConcept C186060115 @default.
• W2979958958 hasConcept C192562407 @default.
• W2979958958 hasConcept C31972630 @default.

• next