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- W2980041593 abstract "To select the mutants of HPV type 16 E6 and E7 genes suitable for construction of vaccine for treatment of cervical cancer, the transforming activities of the mutants of HPV16 E6 and E7 genes were studied. Ballb/c 3T3 cells were transfected with HPV16 wild and mutated E6 and E7 genes. The G418 - resistant clones from each group were examined for their anchorage - independent growth in soft agar and tumorigenicity in nude mice. The results showed that Balb/c 3T3 cell lines transfected with wE6, wE7 and wE6wE7 genes exhibited anchorage - independent growth in soft agar and tumorigienicity in nude mice. Whereas the Balb/c 3T3 cell lines transfected with mE6(50G), mE7 - 1(24G26G), mE7 - 3(24G26G67R) and mE6mE7 - 1 lost the ability of anchorage - independent growth in soft agar and tumorigenicity in nude mice. The mutants mE6 (50G), mE7 - 1 (24G26G) and mE7 - 3(24G26G67R) could be used as the candidate antigens to develop therapeutic vaccine of HPV16 associated tumors." @default.
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- W2980041593 date "2002-01-01" @default.
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- W2980041593 title "Transforming Activities of the Mutants of HPV16 E6 and E7 Genes for Balb/c 3T3 Cells" @default.
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