Matches in SemOpenAlex for { <https://semopenalex.org/work/W2980121198> ?p ?o ?g. }
- W2980121198 endingPage "84" @default.
- W2980121198 startingPage "75" @default.
- W2980121198 abstract "We found that nuclear envelopes stabilize against surfactants in the presence of ethylene glycol (EG). We, therefore, developed a novel subcellular fractionation approach for proteomics using RIPA buffer containing EG and phase transfer surfactants. This method involves separating the cells into the cytoplasm, organelles, and nucleus, including intermediate filaments without ultracentrifugation. These fractions are directly applicable to sample preparation for shotgun proteomics as they have no mass spectrometry (MS)-incompatible chemicals, whereas those separated by traditional fractionation protocols require desalting. This protocol is successfully applied to subcellular fractionation with only 3.5 × 105 cells. Here, it was combined with phosphoproteomics and proteomics to identify phosphorylation sites regulating protein subcellular localization. In total, 59 phosphorylation sites on 42 phosphopeptides and 32 proteins showing different enrichment patterns between phosphoproteomics and the corresponding proteomics were identified, which are potential candidate sites to regulate the protein subcellular localization, including serine 706 on CD44 and serine 22 on lamin A/C." @default.
- W2980121198 created "2019-10-18" @default.
- W2980121198 creator A5001763350 @default.
- W2980121198 creator A5015575713 @default.
- W2980121198 creator A5028248102 @default.
- W2980121198 creator A5031965892 @default.
- W2980121198 creator A5038913661 @default.
- W2980121198 date "2019-10-10" @default.
- W2980121198 modified "2023-10-12" @default.
- W2980121198 title "Mass Spectrometry-Compatible Subcellular Fractionation for Proteomics" @default.
- W2980121198 cites W1205458192 @default.
- W2980121198 cites W1970893579 @default.
- W2980121198 cites W1974983216 @default.
- W2980121198 cites W1985585224 @default.
- W2980121198 cites W1986656413 @default.
- W2980121198 cites W2003236418 @default.
- W2980121198 cites W2014695920 @default.
- W2980121198 cites W2020018374 @default.
- W2980121198 cites W2020191371 @default.
- W2980121198 cites W2028481967 @default.
- W2980121198 cites W2028930390 @default.
- W2980121198 cites W2032882035 @default.
- W2980121198 cites W2036710186 @default.
- W2980121198 cites W2039231543 @default.
- W2980121198 cites W2046973476 @default.
- W2980121198 cites W2052574358 @default.
- W2980121198 cites W2067780683 @default.
- W2980121198 cites W2071167287 @default.
- W2980121198 cites W2078533553 @default.
- W2980121198 cites W2080352379 @default.
- W2980121198 cites W2083682157 @default.
- W2980121198 cites W2094238516 @default.
- W2980121198 cites W2101513682 @default.
- W2980121198 cites W2102690059 @default.
- W2980121198 cites W2103770181 @default.
- W2980121198 cites W2109025098 @default.
- W2980121198 cites W2114915170 @default.
- W2980121198 cites W2119445385 @default.
- W2980121198 cites W2137983743 @default.
- W2980121198 cites W2139412961 @default.
- W2980121198 cites W2140946052 @default.
- W2980121198 cites W2156355974 @default.
- W2980121198 cites W2165962849 @default.
- W2980121198 cites W2169359398 @default.
- W2980121198 cites W2172341829 @default.
- W2980121198 cites W2191641478 @default.
- W2980121198 cites W2288790541 @default.
- W2980121198 cites W2340807121 @default.
- W2980121198 cites W2418435728 @default.
- W2980121198 cites W2475916508 @default.
- W2980121198 cites W2557925011 @default.
- W2980121198 cites W2622390363 @default.
- W2980121198 cites W2755452354 @default.
- W2980121198 cites W2794340145 @default.
- W2980121198 cites W40997965 @default.
- W2980121198 doi "https://doi.org/10.1021/acs.jproteome.9b00347" @default.
- W2980121198 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/31599158" @default.
- W2980121198 hasPublicationYear "2019" @default.
- W2980121198 type Work @default.
- W2980121198 sameAs 2980121198 @default.
- W2980121198 citedByCount "18" @default.
- W2980121198 countsByYear W29801211982020 @default.
- W2980121198 countsByYear W29801211982021 @default.
- W2980121198 countsByYear W29801211982022 @default.
- W2980121198 countsByYear W29801211982023 @default.
- W2980121198 crossrefType "journal-article" @default.
- W2980121198 hasAuthorship W2980121198A5001763350 @default.
- W2980121198 hasAuthorship W2980121198A5015575713 @default.
- W2980121198 hasAuthorship W2980121198A5028248102 @default.
- W2980121198 hasAuthorship W2980121198A5031965892 @default.
- W2980121198 hasAuthorship W2980121198A5038913661 @default.
- W2980121198 hasConcept C104317684 @default.
- W2980121198 hasConcept C104397665 @default.
- W2980121198 hasConcept C11960822 @default.
- W2980121198 hasConcept C140051345 @default.
- W2980121198 hasConcept C162356407 @default.
- W2980121198 hasConcept C168240541 @default.
- W2980121198 hasConcept C185592680 @default.
- W2980121198 hasConcept C190062978 @default.
- W2980121198 hasConcept C27239569 @default.
- W2980121198 hasConcept C2776879804 @default.
- W2980121198 hasConcept C2779933727 @default.
- W2980121198 hasConcept C41625074 @default.
- W2980121198 hasConcept C43617362 @default.
- W2980121198 hasConcept C46111723 @default.
- W2980121198 hasConcept C55493867 @default.
- W2980121198 hasConcept C6675166 @default.
- W2980121198 hasConcept C68289359 @default.
- W2980121198 hasConcept C80311884 @default.
- W2980121198 hasConcept C87325107 @default.
- W2980121198 hasConcept C97029542 @default.
- W2980121198 hasConcept C97428945 @default.
- W2980121198 hasConceptScore W2980121198C104317684 @default.
- W2980121198 hasConceptScore W2980121198C104397665 @default.
- W2980121198 hasConceptScore W2980121198C11960822 @default.
- W2980121198 hasConceptScore W2980121198C140051345 @default.
- W2980121198 hasConceptScore W2980121198C162356407 @default.
- W2980121198 hasConceptScore W2980121198C168240541 @default.